Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gelsolin Double Nickase Plasmid (h): sc-401005-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gelsolin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gelsolin Double Nickase Plasmid (h) and Gelsolin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gelsolin Antibody (F-5): sc-514502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gelsolin Double Nickase Plasmid (h) | sc-401005-NIC | 20 µg | $410.00 | |||
Gelsolin Double Nickase Plasmid (h2) | sc-401005-NIC-2 | 20 µg | $410.00 |
GSN encodes gelsolin, a Ca²⁺-regulated actin-binding protein that severs and caps actin filaments to remodel the cytoskeleton and control cell shape, adhesion, and motility. By regulating actin dynamics, gelsolin influences processes such as endocytosis, membrane trafficking, and apoptosis, and intersects with signaling networks including phosphoinositide and Rho-family GTPase pathways that coordinate cytoskeletal turnover. Altered gelsolin activity or expression has been linked to dysregulated cell migration and invasion phenotypes and is studied across contexts including inflammation, neurobiology, and cardiovascular remodeling. Pathogenic variants in GSN are also associated with amyloidogenic protein misfolding disorders, supporting its relevance in protein homeostasis and extracellular matrix–related pathology.
Gelsolin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.