Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GDF-8 Double Nickase Plasmid (h): sc-400693-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GDF-8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GDF-8 Double Nickase Plasmid (h) and GDF-8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MSTN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GDF-8 Antibody (1L4): sc-134345
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GDF-8 Double Nickase Plasmid (h) | sc-400693-NIC | 20 µg | $410.00 | |||
GDF-8 Double Nickase Plasmid (h2) | sc-400693-NIC-2 | 20 µg | $410.00 |
MSTN encodes growth differentiation factor 8 (GDF-8, myostatin), a secreted TGF-β superfamily ligand that restrains skeletal muscle growth by limiting myoblast proliferation and differentiation. GDF-8 signals primarily through activin type II receptors to activate SMAD2/3-dependent transcriptional programs and cross-talk with PI3K–AKT–mTOR and ubiquitin–proteasome pathways that regulate protein synthesis and degradation. Altered MSTN/GDF-8 activity has been associated with muscle hypertrophy or wasting phenotypes and is frequently studied in the context of sarcopenia, cachexia, muscular dystrophy models, and metabolic regulation. In vitro, MSTN perturbation informs mechanisms of myogenesis, extracellular signaling, and fiber-type remodeling in human cells.
GDF-8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MSTN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MSTN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MSTN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MSTN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.