GDF-10 CRISPR/Cas9 KO Plasmid (h): sc-406826
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GDF-10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GDF-10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GDF-10 CRISPR/Cas9 KO Plasmid (h) | sc-406826 | 20 µg | $397.00 |
Overview
GDF10 encodes growth differentiation factor 10 (GDF-10), a secreted TGF-β superfamily ligand implicated in regulation of cellular differentiation, tissue patterning, and extracellular matrix remodeling. GDF-10 signals through serine/threonine kinase receptors to engage canonical SMAD-dependent transcriptional programs and intersect with broader TGF-β/BMP pathway crosstalk that shapes cell fate decisions and stromal responses. Altered GDF10 expression has been linked in the literature to dysregulated developmental processes and aberrant remodeling programs relevant to fibrotic and tumor-associated microenvironments. In vitro, GDF-10 is frequently studied for its effects on lineage commitment, migration, and context-specific growth control in human cell models.
GDF-10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GDF10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GDF10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GDF10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GDF-10 protein expression.
This CRISPR knockout system enables efficient generation of GDF10-deficient cell models for investigation of GDF-10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GDF10 exon(s) critical for GDF-10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GDF10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GDF-10 CRISPR/Cas9 KO Plasmid (h) and GDF-10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GDF10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GDF-10 HDR Plasmid (h) and GDF-10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GDF10 homology arms to support homology-directed repair at defined GDF10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.