Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GCS-β-1 Double Nickase Plasmid (h): sc-403225-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCS-β-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GCS-β-1 Double Nickase Plasmid (h) and GCS-β-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GUCY1B3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GCS-β-1 Antibody (G-3): sc-514183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCS-β-1 Double Nickase Plasmid (h) | sc-403225-NIC | 20 µg | $410.00 | |||
GCS-β-1 Double Nickase Plasmid (h2) | sc-403225-NIC-2 | 20 µg | $410.00 |
GUCY1B3 encodes the β3 subunit of soluble guanylyl cyclase (GCS-β-1), a key receptor for nitric oxide that catalyzes conversion of GTP to cGMP. This NO–sGC–cGMP axis regulates vascular smooth muscle relaxation, platelet function, and neurotransmission through downstream effectors such as cGMP-dependent protein kinase and phosphodiesterases, integrating signals that control cellular tone and redox-responsive signaling. Altered cGMP signaling and sGC subunit composition have been associated with cardiovascular and pulmonary biology, inflammatory responses, and dysregulated hemostasis, making GUCY1B3 a useful target for mechanistic studies of NO-dependent signal transduction. In human cell models, perturbing GUCY1B3 can help dissect how sGC activity contributes to cGMP dynamics and downstream transcriptional and metabolic adaptations.
GCS-β-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GUCY1B3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GUCY1B3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GUCY1B3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GUCY1B3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.