GBP3 Double Nickase Plasmid (h): sc-402792-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBP3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GBP3 Double Nickase Plasmid (h) and GBP3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GBP3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBP3 Double Nickase Plasmid (h) | sc-402792-NIC | 20 µg | $410.00 | |||
GBP3 Double Nickase Plasmid (h2) | sc-402792-NIC-2 | 20 µg | $410.00 |
Guanylate-binding protein 3 (GBP3) is a human interferon-inducible large GTPase that functions as part of cell-intrinsic immunity during pathogen challenge and inflammatory signaling. As a member of the GBP family, it participates in interferon-stimulated gene networks, contributes to antimicrobial defense mechanisms, and can influence innate immune pathways that shape cytokine responses. GBP3 activity is commonly linked to interferon/JAK–STAT-driven transcriptional programs and broader host defense processes such as inflammasome-associated signaling and stress responses. Dysregulated interferon signaling and altered GBP3 expression have been observed in inflammatory and infectious disease contexts, making it relevant for mechanistic studies of immune activation and immune-associated pathology.
GBP3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GBP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GBP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GBP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GBP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.