Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GBP2 Double Nickase Plasmid (m): sc-420501-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GBP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GBP2 Double Nickase Plasmid (m) and GBP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Gbp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GBP2 Double Nickase Plasmid (m) | sc-420501-NIC | 20 µg | $410.00 |
Mouse Gbp2 encodes guanylate-binding protein 2 (GBP2), an interferon-inducible large GTPase that participates in cell-intrinsic immunity. GBP2 is upregulated downstream of type I and type II interferon signaling through JAK–STAT pathways and supports antimicrobial defense by modulating pathogen-restrictive programs, vesicular trafficking, and inflammasome-associated responses. In immune and barrier tissues, GBP2 contributes to inflammatory signaling networks that shape host–pathogen interactions and can influence disease-relevant phenotypes linked to dysregulated interferon responses. Because GBP family members intersect with innate immune sensing and cytokine-driven transcriptional programs, Gbp2 perturbation is useful for dissecting interferon-stimulated gene function in infection, inflammation, and immunometabolism models.
GBP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Gbp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Gbp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Gbp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Gbp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.