GATA-1 Antibody (N1) recommended for detection of GATA-1 of mouse, rat and human origin by WB, IP and IF

GATA-1 Antibody (N1): sc-266

GATA-1 Antibody (N1) is rated 5.0 out of 5 by 9.
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Datasheets

    • GATA-1 Antibody (N1) is a rat monoclonal IgG2a provided at 200 µg/ml
    • raised against bacterially expressed murine GATA-1
    • Anti-GATA-1 Antibody (N1) is recommended for detection of GATA-1 of mouse, rat and human origin by WB, IP and IF; inhibits DNA binding in gel shift studies
    • Anti-GATA-1 Antibody (N1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-266 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GATA-1 (N1): sc-266.
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When should the TransCruz reagent sc-266 X be used instead of the standard form sc-266?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Tech Service
Date published: 2017-01-31
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Rated 5 out of 5 by from Nice band We used this antibody for a western blot and got a very clean band
Date published: 2016-12-23
Rated 5 out of 5 by from This antibody worked well for IP This antibody worked well for IP, published with Cos, HPCs (human) and mouse lymphoma cells. -SCBT Publication Review
Date published: 2015-04-20
Rated 5 out of 5 by from This GATA This GATA-1 antiobody (N1) was useful in immunohistochemistry using human hematopoietic cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Publishable data obtained using product sc Publishable data obtained using product sc-266 in immunohistochemistry on formalin fixed, paraffin embedded (FFPE) mouse gonads. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Works well in Western Blot using human hematopoietic, HPCs, erythroid, K-562, MEL, UT-7, Jurkat, HeLa, UT-7 5.3, U-937, HL-60 HEL and TF1 cell lines. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Human MEL and umbelical vein endothelial Human MEL and umbelical vein endothelial cells were used in ChIP application successfully and results published. -SCBT Publication Review
Date published: 2015-02-20
Rated 5 out of 5 by from Antibody was used successfully in EMSA using Antibody was used successfully in EMSA using mouse C19 MEL and human Jurkat, HeLa, K-562, ovarian and breast cells. -SCBT Publication Review
Date published: 2015-01-25
Rated 5 out of 5 by from Good Western Blot data with mouse WBB6F1 Good Western Blot data with mouse WBB6F1, C19 MEL, erythroblasts, CCE ES cells, OP9 Stromal, and lymphoma cells. -SCBT Publication Review
Date published: 2015-01-23
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