GAPDH Antibody (8C2): sc-81545

GAPDH Antibody (8C2) is a mouse monoclonal IgG1 antibody that specifically targets human glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GAPDH is widely known for its role in glycolysis, where GAPDH catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, an essential step in energy metabolism. Beyond glycolysis, GAPDH is involved in various cellular processes, including transcriptional regulation, DNA repair, and apoptosis, making GAPDH valuable for research in cell biology, cancer, and neurodegenerative disorders. Anti-GAPDH antibody (8C2) is particularly useful in detecting GAPDH in contexts such as oxidative stress studies, where GAPDH undergoes post-translational modifications such as S-nitrosylation and oxidation at its catalytic cysteine, which alters enzymatic activity and facilitates nuclear translocation during apoptosis. These modifications also enable GAPDH to form complexes with proteins like the β-amyloid precursor protein and Huntingtin, linking GAPDH to research on Alzheimer′s and Huntington′s diseases. Moreover, GAPDH is overexpressed in certain cancers, including prostate, lung, and liver cancers, making GAPDH (8C2) antibody a crucial tool for oncology research. Additionally, GAPDH monoclonal antibody (8C2) serves as a loading control in western blotting (WB) due to stable expression across various tissues, further broadening applications in protein quantification studies and gene expression analysis.

The preferred GAPDH antibody is GAPDH Antibody (6C5): sc-32233.