Date published: 2025-9-5
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GAPDH Antibody (6C5) - Western Blotting - Image 398219
GAPDH 抗体 (6C5): sc-32233. MOLT-4 (A), Jurkat (B), PC-3 (C), HeLa (D) 和 c4 (E) 全细胞裂解液中 GAPDH 表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG1 BP-CFL 647: sc-533664.
GAPDH 抗体 (6C5): sc-32233. MOLT-4 (A), Jurkat (B), PC-3 (C), HeLa (D) 和 c4 (E) 全细胞裂解液中 GAPDH 表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgG1 BP-CFL 647: sc-533664.

GAPDH 抗体 (6C5): sc-32233

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说明书
  • GAPDH抗体(6C5)是小鼠单克隆IgG1 κ, 在4,773篇文献中引用，规格为100 µg/ml
  • 针对GAPDH从rabbit肌肉纯化
  • 推荐用于 mouse, rat, human， rabbit 和 Xenopus 来源的GAPDH WB, IP 和 IF检测
  • 看 GAPDH (0411): sc-47724，可查看有标记的 GAPDH 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取GAPDH (6C5): sc-32233的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是GAPDH Antibody (6C5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与GAPDH Antibody (6C5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GAPDH抗体（6C5）是一种小鼠单克隆IgG1 kappa轻链抗体，针对从兔肌肉中纯化的GAPDH，在应用中（如蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF））对小鼠、大鼠、人类和兔物种均具有反应性。甘油醛-3-磷酸脱氢酶（GAPDH）是糖酵解中的一种关键酶，在有无机磷酸和NAD⁺的情况下催化甘油醛-3-磷酸向1,3-双磷酸甘油酯的可逆氧化磷酸化，这是产生细胞能量的碳水化合物代谢的关键步骤。除了代谢作用外，GAPDH还具有多种功能，参与转录激活、RNA结合、核RNA输出、DNA复制和修复，凸显其在各种细胞过程中的重要性。GAPDH主要存在于细胞质中，但也可以转移到细胞核中，尤其是在细胞凋亡期间，这表明它参与了细胞死亡的途径。翻译后修饰，如S-亚硝基化、磷酸化、乙酰化、泛素化和ADP-核糖化，可以调节GAPDH的活性和定位，从而影响细胞凋亡和神经退行性疾病等过程。在肝癌、肺癌和前列腺癌中，GAPDH的表达水平较高，这使得GAPDH成为肿瘤学研究中一种非常有价值的生物标志物。此外，GAPDH与神经退行性疾病相关的神经元蛋白相互作用，包括β-淀粉样蛋白前体蛋白和亨廷顿蛋白，这强调了GAPDH在神经学研究中的重要性。GAPDH (6C5)抗体是研究人员进行代谢、癌症生物学和神经退行性疾病高级研究不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAPDH 抗体 (6C5) 参考文献:

  1. 工程糖酵解甘油醛-3-磷酸脱氢酶与 NAD+ 烟酸酰胺的反构象结合，但不会发生 A 型特异性氢化物转移。  |  Eyschen, J., et al. 1999. Arch Biochem Biophys. 364: 219-27. PMID: 10190977
  2. 对一种古老蛋白质的新认识：哺乳动物甘油醛-3-磷酸脱氢酶的功能多样性。  |  Sirover, MA. 1999. Biochim Biophys Acta. 1432: 159-84. PMID: 10407139
  3. 甘油醛-3-磷酸脱氢酶与细胞凋亡  |  Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
  4. 甘油醛-3-磷酸脱氢酶在神经变性和细胞凋亡信号转导中的作用。  |  Tatton, WG., et al. 2000. J Neural Transm Suppl. 77-100. PMID: 11205159
  5. 正位胰腺肿瘤模型细胞系的转录谱分析揭示了转移相关基因。  |  Tarbé, N., et al. 2001. Anticancer Res. 21: 3221-8. PMID: 11848476
  6. 甘油醛-3-磷酸脱氢酶细胞内结构和功能的改变：神经退行性疾病的共同表型？  |  Mazzola, JL. and Sirover, MA. 2002. Neurotoxicology. 23: 603-9. PMID: 12428732
  7. 病理晚期人类前列腺癌中甘油醛-3-磷酸脱氢酶基因表达增加。  |  Rondinelli, RH., et al. 1997. Prostate Cancer Prostatic Dis. 1: 66-72. PMID: 12496918
  8. GAPDH作为管家基因：在72个人体组织中分析GAPDH mRNA的表达。  |  Barber, RD., et al. 2005. Physiol Genomics. 21: 389-95. PMID: 15769908
  9. 人类核尿嘧啶 DNA 糖基化酶是甘油醛-3-磷酸脱氢酶的 37-kDa 亚基。  |  Meyer-Siegler, K., et al. 1991. Proc Natl Acad Sci U S A. 88: 8460-4. PMID: 1924305
  10. GAPDH：一种具有罕见功能的常见酶。  |  Nicholls, C., et al. 2012. Clin Exp Pharmacol Physiol. 39: 674-9. PMID: 21895736
  11. 神经母细胞瘤中的GAPDH：在代谢和存活中的作用。  |  Cornett, K., et al. 2022. Front Oncol. 12: 979683. PMID: 36267982
  12. GAPDH：一种常见的管家基因，在泛癌中具有致癌作用。  |  Wang, J., et al. 2023. Comput Struct Biotechnol J. 21: 4056-4069. PMID: 37664172

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH 抗体 (6C5)

sc-32233
100 µg/ml
$316.00

GAPDH (6C5): m-IgG Fc BP-HRP 套装

sc-528443
100 µg Ab; 10 µg BP
$354.00

GAPDH (6C5): m-IgGκ BP-HRP 套装

sc-520821
100 µg Ab, 40 µg BP
$354.00

GAPDH (6C5): m-IgG1 BP-HRP 套装

sc-542934
100 µg Ab; 20 µg BP
$354.00

hello to whom it may concern, I was looking for anti-GAPDH (sc-36502) on the website but I did not find it

Asked by: DinaAli
Thank you for your question. sc-36502 has been discontinued. Here are the GAPDH antibodies we offer : https://www.scbt.com/browse/gapdh-Antibodies/_/N-1klthpg?Nr=AND%28OR%28product.catalogId%3AscbtCatalog%29%2Cproduct.discontinuedFlag%3A0%2Cproduct.siteId%3AscbtSite%2Cproduct.webDisplayFlag%3A1%2Cproduct.suppressDisplayFlag%3A0%29&autoSuggest=true&dataType=json
Answered by: BlakeJ
Date published: 2023-02-23

I bought this antibody through a distributor for Immunoprecipitation as guaranteed by SCBT and got nothing. Can I get other antibody sample for IP?

Asked by: it doesnt work
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Yanru Tao
Date published: 2022-09-05

Can this mouse monoclonal antibody be used to detect GAPDH of e. Coli origin?

Asked by: Sarip2
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Hi there! Can I get a FREE 10 µg sample of GAPDH (6C5): sc-32233? Thanks a lot!

Asked by: Nanalucy
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Can this mouse monoclonal antibody be used to detect GAPDH of equine origin?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service
Date published: 2016-12-13

Is this antibody suitable for detecting GAPDH dimmers and tetramers in WB?

Asked by: avale
Thank you for your inquiry. This antibody has been tested for the detection of GAPDH in denaturing conditions, and we have not specifically tested it for the detection of dimers or tetramers. It is warrantied for Western blot applications. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-10-25
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Rated 5 out of 5 by from 真的真的很棒此抗體具高靈敏度與良好專一性，背景低、操作方便，適用多種樣本，實驗穩定可靠，反應表現優異!!!!!!
Date published: 2025-07-18
Rated 5 out of 5 by from Funziona egregiamenteOttimo per la rilevazione della GAPDH in linee cellulari tumorali, come MCF7, HeLa e U2OS
Date published: 2025-07-13
Rated 5 out of 5 by from Great reliable Anti-GAPDH antibody for Westernssc-GADPH antibody is very reliable and very strong. at a dilution of 1:1000, the bands are quite strong.
Date published: 2025-03-18
Rated 5 out of 5 by from Works great for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:3000 for overnight @ 4oC.
Date published: 2024-12-10
Rated 4 out of 5 by from Works well for western blotWe tried this antibody for standard western blot analysis on mouse brain samples. It worked well, as a first test we tried 1:500 but it can be used more diluted.
Date published: 2024-10-24
Rated 5 out of 5 by from Great for western blottingThis antibody is the most useful in Western blotting.
Date published: 2024-08-17
Rated 5 out of 5 by from 很好用购买的gapdh很好用、一下子就显出来~推荐推荐推荐~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Date published: 2024-04-03
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
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GAPDH 抗体 (6C5) is rated 4.7 out of 5 by 95.
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