GAPDH Antibody (1D4): sc-59540
- GAPDH Antibody (1D4) is a mouse monoclonal IgM κ, cited in 140 publications, provided at 100 µg/ml
- raised against GAPDH of porcine origin
- recommended for detection of GAPDH of mouse, rat, human, bovine and porcine origin by WB, IP and IF
- See GAPDH (0411): sc-47724 for GAPDH antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GAPDH Antibody (1D4). This work is in progress.
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SEE ALSO...
GAPDH Antibody (1D4) is a mouse monoclonal IgM kappa light chain antibody raised against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of porcine origin, with reactivity against mouse, rat, human, bovine, and porcine proteins. Anti-GAPDH antibody (1D4) detects GAPDH through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) and is offered in a non-conjugated form. Structurally, GAPDH is a homotetramer, composed of four identical subunits, each featuring an active site for catalysis and a binding site for NAD⁺, which are critical for its role in glycolysis. This quaternary structure not only ensures efficient catalytic activity but also enables cooperative interactions between subunits, enhancing the enzyme′s stability and functional precision. The tetrameric arrangement is vital for GAPDH′s enzymatic function, as alterations or disruptions in this structure can impair glycolytic efficiency and energy production. Furthermore, GAPDH′s structure facilitates diverse non-metabolic roles, including interactions with DNA and RNA, nuclear localization during apoptosis, and binding to proteins implicated in neurodegenerative disorders. Post-translational modifications such as S-nitrosylation and acetylation influence the structural integrity and localization of GAPDH, further emphasizing the functional importance of its architecture. GAPDH monoclonal antibody (1D4) provides a reliable means of studying the structural and functional aspects of GAPDH, contributing to research in cellular metabolism, cancer progression, neurodegeneration, and apoptotic mechanisms.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAPDH Antibody (1D4) | sc-59540 | 100 µg/ml | $322.00 |