GAPDH-2 Antibody (2E3-2E10): sc-293335

GAPDH-2 Antibody (2E3-2E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects GAPDH-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GAPDH-2 (2E3-2E10) antibody is offered in a non-conjugated form, allowing for versatile experimental design. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays a crucial role in cellular metabolism by catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, a key step in glycolysis that generates energy in the form of nicotinamide adenine dinucleotide (NAD). Beyond energy metabolism, GAPDH is involved in various cellular processes, including DNA repair and transcriptional regulation, highlighting GAPDH′s importance in maintaining cellular homeostasis. GAPDH expression is often upregulated in various cancers, including liver, lung, and prostate cancers. During apoptosis, GAPDH translocates to the nucleus, where GAPDH interacts with proteins associated with neurodegenerative disorders, such as the beta-amyloid precursor and Huntingtin, further underscoring GAPDH′s multifaceted role in both normal physiology and disease.

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GAPDH-2 Antibody (2E3-2E10) References:

1. Engineered glycolytic glyceraldehyde-3-phosphate dehydrogenase binds the anti conformation of NAD+ nicotinamide but does not experience A-specific hydride transfer.  |  Eyschen, J., et al. 1999. Arch Biochem Biophys. 364: 219-27. PMID: 10190977
2. New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase.  |  Sirover, MA. 1999. Biochim Biophys Acta. 1432: 159-84. PMID: 10407139
3. Glyceraldehyde-3-phosphate dehydrogenase and apoptosis.  |  Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
4. Glyceraldehyde-3-phosphate dehydrogenase in neurodegeneration and apoptosis signaling.  |  Tatton, WG., et al. 2000. J Neural Transm Suppl. 77-100. PMID: 11205159
5. Transcriptional profiling of cell lines derived from an orthotopic pancreatic tumor model reveals metastasis-associated genes.  |  Tarbé, N., et al. 2001. Anticancer Res. 21: 3221-8. PMID: 11848476
6. Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders?  |  Mazzola, JL. and Sirover, MA. 2002. Neurotoxicology. 23: 603-9. PMID: 12428732
7. Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in late pathological stage human prostate cancer.  |  Rondinelli, RH., et al. 1997. Prostate Cancer Prostatic Dis. 1: 66-72. PMID: 12496918
8. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase.  |  Meyer-Siegler, K., et al. 1991. Proc Natl Acad Sci U S A. 88: 8460-4. PMID: 1924305
9. Hypobaric hypoxia and reoxygenation induce proteomic profile changes in the rat brain cortex.  |  Hernández, R., et al. 2013. Neuromolecular Med. 15: 82-94. PMID: 22961459
10. The temporal expression of adipokines during spinal fusion.  |  Virk, S., et al. 2017. Spine J. 17: 1897-1906. PMID: 28647583
11. [Effects of andrographolide on the expression of procoagulant and fibrinolytic inhibition factors in rat type II alveolar epithelial cells stimulated by lipopolysaccharide].  |  Yang, G., et al. 2021. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 33: 155-160. PMID: 33729132
12. [Mechanism of Notch3 signaling pathway regulating the differentiation of aortic dissection vascular stem cells into smooth muscle cells].  |  Han, Y., et al. 2023. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 35: 503-508. PMID: 37308231

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAPDH-2 Antibody (2E3-2E10) has 101 citations in a variety of scientific publications.

Primary antibodies like Anti-GAPDH-2 Antibody (2E3-2E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAPDH-2 Antibody (2E3-2E10) Specifications

Alternate Product Name: GAPDH-2

Product Clone Name: 2E3-2E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-408

Epitope Target: GAPDH-2

Epitope Species: human

Product Reactivity/Detection: GAPDH-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 101 citations in various scientific publications

Available Conjugates/Forms for GAPDH-2 Antibody (2E3-2E10): N/A