γ-crystallin Antibody (F-4): sc-514201

γ-crystallin Antibody (F-4) is a mouse monoclonal IgG1 antibody that detects γ-crystallin in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γ-crystallin is a crucial structural protein found predominantly in the lens of the vertebrate eye, where γ-crystallin plays a vital role in maintaining lens transparency and refractive index, essential for proper vision. γ-crystallin is characterized by its monomeric form, which typically lacks connecting peptides and terminal extensions, allowing for a compact structure that is critical for its function. The γ-crystallin family includes several closely related isoforms, such as γA, γB, γC, γD, γE, γF, and γG, as well as γN and γS, each of which is differentially regulated during development. Alterations in the expression or structure of γ-crystallins can lead to cataract formation, highlighting γ-crystallin′s significance in ocular health. anti-γ-crystallin antibody (F-4) is an invaluable tool for researchers studying the molecular mechanisms underlying lens transparency and the pathogenesis of cataracts, providing insights into both normal physiology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

γ-crystallin Antibody (F-4) References:

1. Chaperone-like activity of a synthetic peptide toward oxidized gamma-crystallin.  |  Kumar, RS. and Sharma, KK. 2000. J Pept Res. 56: 157-64. PMID: 11007272
2. Antagonistic action of Six3 and Prox1 at the gamma-crystallin promoter.  |  Lengler, J., et al. 2001. Nucleic Acids Res. 29: 515-26. PMID: 11139622
3. Altered Na,K-ATPase pattern in gamma-crystallin mutant mice.  |  Moseley, A., et al. 2002. Invest Ophthalmol Vis Sci. 43: 1517-9. PMID: 11980868
4. DNA methylation as a regulatory mechanism in rat gamma-crystallin gene expression.  |  Peek, R., et al. 1991. Nucleic Acids Res. 19: 77-83. PMID: 2011513
5. Mutation screening and genotype phenotype correlation of α-crystallin, γ-crystallin and GJA8 gene in congenital cataract.  |  Kumar, M., et al. 2011. Mol Vis. 17: 693-707. PMID: 21423869
6. Electrostatic origin of in vitro aggregation of human γ-crystallin.  |  Mohr, BG., et al. 2013. J Chem Phys. 139: 121914. PMID: 24089726
7. EGCG prevents tryptophan oxidation of cataractous ocular lens human γ-crystallin in presence of H2O2.  |  Chaudhury, S., et al. 2015. Int J Biol Macromol. 77: 287-92. PMID: 25841365
8. The Structural Alteration and Aggregation of Bovine Lens Gamma-Crystallin by Homocysteinylation; The Pathomechanism Underlying Cataract Development During Hyperhomocysteinimia.  |  Hajjari, S., et al. 2016. Protein Pept Lett. 23: 78-86. PMID: 26548860
9. The evolution of lenticular proteins: the beta- and gamma-crystallin super gene family.  |  Lubsen, NH., et al. 1988. Prog Biophys Mol Biol. 51: 47-76. PMID: 3064189
10. Opacification of gamma-crystallin solutions from calf lens in relation to cold cataract formation.  |  Siezen, RJ., et al. 1985. Proc Natl Acad Sci U S A. 82: 1701-5. PMID: 3856852

The preferred γ-crystallin antibody is γ-crystallin Antibody (B-5): sc-365256.