γ-crystallin Antibody (B-5): sc-365256

γ-crystallin Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects gamma-crystallin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-gamma-crystallin antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gamma-crystallins play a crucial role in maintaining the transparency and refractive index of the vertebrate eye lens, which is essential for proper vision. These proteins are primarily located in the lens fibers, where they contribute to the lens′s structural integrity and optical properties. The unique structural characteristics of gamma-crystallins, which typically exist as monomers and lack connecting peptides and terminal extensions, allow them to pack tightly together, minimizing light scattering and ensuring clarity. Additionally, the gamma-crystallin family includes several closely related proteins, such as γA, γB, γC, γD, γE, γF, and γG, as well as γN and γS, each of which is differentially regulated during development. Understanding the function and location of gamma-crystallins is vital, as alterations in their expression or structure can lead to cataract formation, highlighting their importance in ocular health.

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γ-crystallin Antibody (B-5) References:

1. Crystal cataracts: human genetic cataract caused by protein crystallization.  |  Pande, A., et al. 2001. Proc Natl Acad Sci U S A. 98: 6116-20. PMID: 11371638
2. Lens crystallins and their microbial homologs: structure, stability, and function.  |  Jaenicke, R. and Slingsby, C. 2001. Crit Rev Biochem Mol Biol. 36: 435-99. PMID: 11724156
3. Expression and regulation of alpha-, beta-, and gamma-crystallins in mammalian lens epithelial cells.  |  Wang, X., et al. 2004. Invest Ophthalmol Vis Sci. 45: 3608-19. PMID: 15452068
4. Functional Role of γ-Crystallin N in the Auditory Hindbrain.  |  Hartwich, H., et al. 2016. PLoS One. 11: e0161140. PMID: 27517863
5. Acquired Disorder and Asymmetry in a Domain-Swapped Model for γ-Crystallin Aggregation.  |  Sagar, V. and Wistow, G. 2022. J Mol Biol. 434: 167559. PMID: 35341744
6. Atomistic modeling of liquid-liquid phase equilibrium explains dependence of critical temperature on γ-crystallin sequence.  |  Qin, S. and Zhou, HX. 2023. Commun Biol. 6: 886. PMID: 37644195
7. Interaction of βL- and γ-Crystallin with Phospholipid Membrane Using Atomic Force Microscopy.  |  Khadka, NK., et al. 2023. Int J Mol Sci. 24: PMID: 37958704
8. Cell Penetrability of a γ-Crystallin Peptide Fragment from the Discarded Cataractous Eye Emulsion.  |  Chowdhury, P., et al. 2024. ACS Omega. 9: 14840-14848. PMID: 38585046
9. Regulation of expression within a gene family. The case of the rat gammaB- and gammaD-crystallin promoters.  |  Klok, EJ., et al. 1998. J Biol Chem. 273: 17206-15. PMID: 9642290
10. Purification of gamma-crystallin from human lenses by acetone precipitation method.  |  Srivastava, OP. and Srivastava, K. 1998. Curr Eye Res. 17: 1074-81. PMID: 9846626
11. Degradation of gamma D- and gamma s-crystallins in human lenses.  |  Srivastava, OP. and Srivastava, K. 1998. Biochem Biophys Res Commun. 253: 288-94. PMID: 9878530
12. Progressive juvenile-onset punctate cataracts caused by mutation of the gammaD-crystallin gene.  |  Stephan, DA., et al. 1999. Proc Natl Acad Sci U S A. 96: 1008-12. PMID: 9927684

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ-crystallin Antibody (B-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-γ-crystallin Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ-crystallin Antibody (B-5) Specifications

Alternate Product Name: γ-crystallin

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 17-49

Epitope Target: γ-crystallin

Epitope Species: human

Product Reactivity/Detection: γ-crystallin A, B, C, D, E, F

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for γ-crystallin Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin