G_{α q} Antibody (10): sc-136181

Gα q Antibody (10) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 22-31 of human Gα q, reactive with mouse, rat, human, and canine species, and validated for applications including western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Gα q, a member of the heterotrimeric G protein family, plays a pivotal role in intracellular signaling by coupling cell surface receptors to downstream effector molecules. These G proteins are composed of α, β, and γ subunits, with the α subunit, such as Gα q, responsible for binding and hydrolyzing GTP and directly interacting with effectors to modulate cellular responses. Gα q is integral to the Gq signaling pathway, which activates phospholipase C (PLC) upon stimulation by various extracellular signals, such as hormones, neurotransmitters, and sensory stimuli. This activation leads to the production of second messengers, including inositol trisphosphate (IP3) and diacylglycerol (DAG), which regulate processes like calcium release and protein kinase activation. Gα q is highly expressed in tissues such as the brain and lungs, where Gα q contributes to critical functions in neural signaling and respiratory regulation, and is also found at lower levels in other tissues. Dysregulation of Gα q-mediated pathways is implicated in a range of pathological conditions, including cardiovascular diseases, neurological disorders, and certain cancers, underscoring Gα q importance in maintaining cellular homeostasis. Anti-Gα q antibody (10) is a reliable tool for studying the structure, function, and regulatory mechanisms of this essential G protein subunit in both physiological and pathological contexts. Gα q (10) monoclonal antibody is available in both non-conjugated and conjugated forms to support a wide range of experimental applications.

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G_{α q} Antibody (10) References:

1. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
2. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
3. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
4. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
5. G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.  |  Strathmann, M. and Simon, MI. 1990. Proc Natl Acad Sci U S A. 87: 9113-7. PMID: 2123549
6. ADRA1A-Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP.  |  Rahbani, JF., et al. 2022. Nat Metab. 4: 1459-1473. PMID: 36344764
7. Control of Gαq signaling dynamics and GPCR cross-talk by GRKs.  |  Xiang, G., et al. 2022. Sci Adv. 8: eabq3363. PMID: 36427324
8. Unraveling allostery within the angiotensin II type 1 receptor for Gαq and β-arrestin coupling.  |  Cao, Y., et al. 2023. Sci Signal. 16: eadf2173. PMID: 37552769
9. The mechanism of Gαq regulation of PLCβ3-catalyzed PIP2 hydrolysis.  |  Falzone, ME. and MacKinnon, R. 2023. Proc Natl Acad Sci U S A. 120: e2315011120. PMID: 37991948
10. Recommended Tool Compounds: Application of YM-254890 and FR900359 to Interrogate Gαq/11-Mediated Signaling Pathways.  |  Voss, JH. 2023. ACS Pharmacol Transl Sci. 6: 1790-1800. PMID: 38093837
11. Selective optogenetic inhibition of Gαq or Gαi signaling by minimal RGS domains disrupts circuit functionality and circuit formation.  |  Lockyer, JL., et al. 2024. Proc Natl Acad Sci U S A. 121: e2411846121. PMID: 39190348
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α q} Antibody (10) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α q} Antibody (10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α q} Antibody (10) Specifications

Alternate Product Name: G protein subunit GNAQ

Product Clone Name: 10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 22-31

Epitope Target: G_{α q}

Epitope Species: human

Product Reactivity/Detection: G_{α q}

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for G_{α q} Antibody (10): AC: Agarose, HRP: Horseradish Peroxidase