GalNAc-T6 Antibody (Y5J): sc-100755

GalNAc-T6 Antibody (Y5J) is a mouse monoclonal IgG1 kappa light chain antibody that detects GalNAc-T6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-GalNAc-T6 antibody (Y5J) is available as a non-conjugated format. The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes, to which GalNAc-T6 belongs, plays a crucial role in initiating mucin-type O-linked glycosylation, a process vital for protein function and cellular communication. GalNAc-T6, also known as GALNT6 (Polypeptide N-acetylgalactosaminyltransferase 6), is a 622 amino acid single-pass type II membrane protein primarily localized in the Golgi apparatus. GalNAc-T6′s structure includes a stem region and a C-terminal ricin/lectin-like domain, which are essential for enzymatic activity. GalNAc-T6 shows high expression in tissues such as the trachea, fibroblasts, and placenta, with lower levels in the brain and pancreas. GalNAc-T6 catalyzes the transfer of an N-acetyl-D-galactosamine residue to serine and threonine residues on target proteins, marking the first step in O-linked oligosaccharide biosynthesis. This enzymatic action is not only critical for normal cellular functions but also has implications in cancer biology, as GalNAc-T6 may serve as a potential marker for breast cancer and participates in the synthesis of oncofetal fibronectin.

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GalNAc-T6 Antibody (Y5J) References:

1. Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy.  |  Bennett, EP., et al. 1999. J Biol Chem. 274: 25362-70. PMID: 10464263
2. Activity of partially purified UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase with different peptide acceptors.  |  Porowska, H., et al. 1999. Acta Biochim Pol. 46: 365-70. PMID: 10547037
3. Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and mammals. One subfamily composed of l(2)35Aa is essential in Drosophila.  |  Schwientek, T., et al. 2002. J Biol Chem. 277: 22623-38. PMID: 11925450
4. The cell-layer- and cell-type-specific distribution of GalNAc-transferases in the ocular surface epithelia is altered during keratinization.  |  Argüeso, P., et al. 2003. Invest Ophthalmol Vis Sci. 44: 86-92. PMID: 12506059
5. UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6 as a new immunohistochemical breast cancer marker.  |  Berois, N., et al. 2006. J Histochem Cytochem. 54: 317-28. PMID: 16260590
6. UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (ppGalNAc-T6) mRNA as a potential new marker for detection of bone marrow-disseminated breast cancer cells.  |  Freire, T., et al. 2006. Int J Cancer. 119: 1383-8. PMID: 16596643

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GalNAc-T6 Antibody (Y5J) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-GalNAc-T6 Antibody (Y5J) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GalNAc-T6 Antibody (Y5J) Specifications

Alternate Product Name: GalNAc-T6

Product Clone Name: Y5J

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 523-623

Epitope Target: GalNAc-T6

Epitope Species: human

Product Reactivity/Detection: GalNAc-T6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for GalNAc-T6 Antibody (Y5J): N/A