GalNAc-T3 Double Nickase Plasmid (h): sc-407682-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GalNAc-T3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GalNAc-T3 Double Nickase Plasmid (h) and GalNAc-T3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GALNT3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GalNAc-T3 Double Nickase Plasmid (h) | sc-407682-NIC | 20 µg | $410.00 | |||
GalNAc-T3 Double Nickase Plasmid (h2) | sc-407682-NIC-2 | 20 µg | $410.00 |
GALNT3 encodes the polypeptide N-acetylgalactosaminyltransferase GalNAc-T3, a Golgi-resident enzyme that initiates mucin-type O-glycosylation by transferring GalNAc to serine/threonine residues on secreted and membrane proteins. This early glycosylation step shapes protein folding, stability, and trafficking, and it modulates cell–cell interactions and receptor signaling through remodeling of the cell surface and extracellular matrix. GalNAc-T3 activity interfaces with pathways governing secretion, epithelial differentiation, and glycoprotein quality control, with downstream effects on adhesion and growth factor responsiveness. Genetic or functional perturbation of GALNT3 is linked to altered phosphate homeostasis and ectopic calcification phenotypes, making it relevant for studies of glycosylation-dependent regulation of endocrine signaling and mineral metabolism.
GalNAc-T3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GALNT3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GALNT3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GALNT3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GALNT3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.