DDIT3/GADD153/CHOP (m): 293T Lysate: sc-120383

The GADD153 (m): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line well-known for its high transfection efficiency. This lysate contains the GADD153 protein, also known as CHOP (C/EBP homologous protein), which is a key regulator of cellular stress responses, particularly in response to endoplasmic reticulum (ER) stress. GADD153 is activated under conditions of cellular stress, leading to the transcriptional activation of genes involved in apoptosis, cell cycle arrest, and stress responses. The protein functions by dimerizing with other transcription factors like C/EBP, modulating their activity and altering gene expression profiles. In research, GADD153 (m): 293T Lysate is used to study the mechanisms of ER stress and the unfolded protein response (UPR). It is particularly valuable for investigating the molecular pathways that lead to GADD153 activation and its role in cellular stress signaling. Researchers utilize this lysate in in vitro assays to examine protein-protein interactions, post-translational modifications, and the transcriptional activity of GADD153. This lysate also aids in screening potential modulators of the UPR pathway, providing insights into how cells respond to stress and maintain homeostasis. Through these applications, the lysate contributes to a deeper understanding of stress response mechanisms in cells.