GABAC Rρ2 Double Nickase Plasmid (h): sc-404955-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAC Rρ2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GABAC Rρ2 Double Nickase Plasmid (h) and GABAC Rρ2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GABRR2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAC Rρ2 Double Nickase Plasmid (h) | sc-404955-NIC | 20 µg | $410.00 | |||
GABAC Rρ2 Double Nickase Plasmid (h2) | sc-404955-NIC-2 | 20 µg | $410.00 |
GABRR2 encodes the human GABA\(_C\) receptor rho2 (GABA\(_C\) Rρ2), a ligand-gated chloride channel of the Cys-loop receptor family that mediates inhibitory neurotransmission. Upon GABA binding, rho2-containing receptors conduct Cl⁻ to shape membrane excitability and synaptic signaling, influencing circuit activity and neuronal information processing. GABA\(_C\) Rρ2 function intersects with broader GABAergic pathways that regulate excitation–inhibition balance and activity-dependent network states. Dysregulation of inhibitory signaling and altered receptor subunit expression patterns are studied in the context of neurological and neuropsychiatric disease mechanisms, supporting ongoing investigation of GABRR2 in brain function and dysfunction.
GABAC Rρ2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GABRR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GABRR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GABRR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GABRR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.