Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GABAA Rβ3 Double Nickase Plasmid (h): sc-403007-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rβ3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GABAA Rβ3 Double Nickase Plasmid (h) and GABAA Rβ3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GABRB3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GABAA Rβ3 Antibody (D-12): sc-376252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rβ3 Double Nickase Plasmid (h) | sc-403007-NIC | 20 µg | $410.00 | |||
GABAA Rβ3 Double Nickase Plasmid (h2) | sc-403007-NIC-2 | 20 µg | $410.00 |
GABRB3 encodes the human GABA_A receptor β3 subunit, a core component of ligand-gated chloride channels that mediate fast inhibitory neurotransmission in the central nervous system. Incorporation of β3 influences receptor assembly, surface trafficking, and pharmacological properties, shaping neuronal excitability through synaptic and extrasynaptic GABAergic signaling. GABA_A receptor activity couples to membrane potential regulation and network oscillations, with downstream effects on synapse maturation and activity-dependent plasticity. Genetic variation or dysregulated expression of GABRB3 has been associated with neurodevelopmental and seizure-related phenotypes, supporting its use in mechanistic studies of inhibitory circuit dysfunction.
GABAA Rβ3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GABRB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GABRB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GABRB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GABRB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.