G3BP1 Double Nickase Plasmid (m): sc-424175-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G3BP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G3BP1 Double Nickase Plasmid (m) and G3BP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting G3bp1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G3BP1 Double Nickase Plasmid (m) | sc-424175-NIC | 20 µg | $410.00 | |||
G3BP1 Double Nickase Plasmid (m2) | sc-424175-NIC-2 | 20 µg | $410.00 |
Mouse G3bp1 encodes G3BP1, a multifunctional RNA-binding protein that integrates mRNA metabolism with cellular stress responses. G3BP1 is a core scaffold of stress granules and influences translation initiation, mRNA stability, and ribonucleoprotein assembly, linking these processes to signaling pathways such as innate immune sensing and kinase-driven stress signaling. Through its roles in cytoplasmic RNA granule dynamics and protein–RNA interactions, altered G3BP1 activity is studied in contexts including neurodegeneration, antiviral responses, and tumor cell adaptation to stress.
G3BP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the G3bp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within G3bp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt G3bp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of G3bp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.