G3BP1 CRISPR/Cas9 KO Plasmid (m): sc-424175
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G3BP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the G3BP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G3BP1 CRISPR/Cas9 KO Plasmid (m) | sc-424175 | 20 µg | $397.00 |
Overview
G3bp1 encodes G3BP1, an RNA-binding protein that nucleates stress granule assembly and coordinates mRNA triage during cellular stress. Through interactions with translation initiation factors and signaling components, G3BP1 links stress responses to pathways regulating mRNA stability, innate immune signaling, and apoptosis, including crosstalk with MAPK and interferon-associated programs. In mouse systems, G3BP1 is commonly studied for its roles in antiviral restriction and modulation of inflammatory signaling, as well as broader effects on proteostasis and cell fate decisions. Dysregulated stress granule dynamics involving G3BP1 have been associated with neurodegeneration and cancer-related phenotypes in experimental models, supporting its relevance to disease-mechanism research.
G3BP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the G3bp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the G3bp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the G3bp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish G3BP1 protein expression.
This CRISPR knockout system enables efficient generation of G3bp1-deficient cell models for investigation of G3BP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting G3bp1 exon(s) critical for G3BP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple G3bp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by G3BP1 CRISPR/Cas9 KO Plasmid (m) and G3BP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the G3bp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by G3BP1 HDR Plasmid (m) and G3BP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by G3bp1 homology arms to support homology-directed repair at defined G3bp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.