G1P3 Double Nickase Plasmid (h): sc-403682-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- G1P3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- G1P3 Double Nickase Plasmid (h) and G1P3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFI6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
G1P3 Double Nickase Plasmid (h) | sc-403682-NIC | 20 µg | $410.00 | |||
G1P3 Double Nickase Plasmid (h2) | sc-403682-NIC-2 | 20 µg | $410.00 |
Human IFI6 encodes the interferon alpha-inducible protein G1P3, a small membrane-associated factor induced downstream of type I interferon signaling through the JAK–STAT pathway. G1P3 has been linked to modulation of mitochondrial integrity and cell survival programs, with reported effects on apoptosis sensitivity and cellular stress responses. As an interferon-stimulated gene, IFI6 participates in innate immune-state regulation and transcriptional programs that reshape antiviral defense and inflammatory signaling networks. Dysregulated IFI6 expression has been observed across multiple disease contexts characterized by chronic interferon activity, including inflammatory conditions and cancers, making it a useful node for mechanistic studies of interferon-driven phenotypes.
G1P3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFI6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFI6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFI6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFI6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.