Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Gα i-1/GNAI1 Double Nickase Plasmid (h): sc-400652-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gα i-1/GNAI1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gα i-1/GNAI1 Double Nickase Plasmid (h) and Gα i-1/GNAI1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GNAI1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gα i-1/GNAI1 Antibody (R4): sc-13533
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gα i-1/GNAI1 Double Nickase Plasmid (h) | sc-400652-NIC | 20 µg | $410.00 | |||
Gα i-1/GNAI1 Double Nickase Plasmid (h2) | sc-400652-NIC-2 | 20 µg | $410.00 |
GNAI1 encodes the inhibitory G protein alpha subunit Gαi-1, a core component of heterotrimeric G proteins that transduce signals from GPCRs to downstream effectors. Upon receptor activation, Gαi-1 suppresses adenylyl cyclase activity to reduce cAMP levels and modulates ion channel function and kinase signaling, including PI3K/AKT and MAPK pathway nodes, through both Gα and Gβγ outputs. These signaling programs regulate proliferation, differentiation, secretion, and chemotactic migration in many cell types. Dysregulated GPCR–G protein coupling and altered cAMP-dependent signaling have been implicated in cancer-associated signaling rewiring, inflammatory responses, and neurological phenotypes, making GNAI1 a useful target for pathway dissection.
Gα i-1/GNAI1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GNAI1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GNAI1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GNAI1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GNAI1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.