Date published: 2026-6-11

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Fyn Antibody (FYN-01): sc-51598

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Datasheets
  • Fyn Antibody (FYN-01) is a mouse monoclonal IgG2b, cited in 2 publications, provided at 100 µg/ml
  • raised against bacterially expressed recombinant fragment, corresponding to amino acids 7-176 of human Fyn
  • recommended for detection of Fyn of mouse, rat and human origin by WB, IP and IF
  • See Fyn (15): sc-434 for Fyn antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Fyn Antibody (FYN-01). This work is in progress.
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Fyn Antibody (FYN-01) is a mouse monoclonal IgG2b light chain antibody that detects Fyn in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Fyn is a member of the Src family of non-receptor tyrosine kinases, which play a crucial role in various cellular processes, including T-cell receptor signaling, cell proliferation, and differentiation. Fyn protein is primarily located in the cytoplasm and can translocate to the plasma membrane, where Fyn interacts with other signaling molecules, thereby influencing cellular responses to external stimuli. This localization is vital for Fyn function, as such positioning allows modulation of pathways essential for immune responses and cellular communication. The Fyn gene, located on chromosome 6q21, encodes a 537 amino acid protein that undergoes various post-translational modifications, including phosphorylation, which regulates activity and interactions with other proteins. Using anti-Fyn antibody (FYN-01) enables researchers to gain insights into Fyn signaling dynamics and implications in health and disease, particularly in T-cell activation and immune response regulation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Fyn Antibody (FYN-01)

sc-51598
100 µg/ml
$322.00

I am using Fyn (FYN-01): sc-51598 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2026, m_6, d_10, h_10CST
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Rated 4 out of 5 by from Positive band visualized in WB in HuT 78Positive band visualized in WB in HuT 78, NIH/3T3, K-562 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-04
Rated 4 out of 5 by from Clear immunofluorescence membrane and nuclearClear immunofluorescence membrane and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-13
Rated 4 out of 5 by from Good band in WB in both human Fyn transfectedGood band in WB in both human Fyn transfected 293 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from Clear band of interest seen in WB in nonClear band of interest seen in WB in non-transfected 293T, human Fyn transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-22
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Fyn Antibody (FYN-01) is rated 4.0 out of 5 by 4.
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