FXR/NR1H4 Double Nickase Plasmid (h): sc-417410-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FXR/NR1H4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FXR/NR1H4 Double Nickase Plasmid (h) and FXR/NR1H4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR1H4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FXR/NR1H4 Antibody (D-3): sc-25309
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FXR/NR1H4 Double Nickase Plasmid (h) | sc-417410-NIC | 20 µg | $410.00 | |||
FXR/NR1H4 Double Nickase Plasmid (h2) | sc-417410-NIC-2 | 20 µg | $410.00 |
NR1H4 encodes farnesoid X receptor (FXR), a bile acid–activated nuclear receptor that functions as a ligand-dependent transcription factor controlling bile acid synthesis, transport, and detoxification. In hepatocytes and enterocytes, FXR coordinates metabolic and inflammatory programs through pathways including FXR–SHP regulation of CYP7A1, induction of bile salt export and uptake transporters, and crosstalk with FGF19/FGF15 signaling. By integrating bile acid homeostasis with lipid and glucose metabolism, FXR influences cellular stress responses, barrier function, and immune modulation in the gut–liver axis. Dysregulated FXR signaling is associated with cholestatic and metabolic liver phenotypes and is studied in contexts such as steatohepatitis, fibrosis progression, and hepatobiliary tumor biology.
FXR/NR1H4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR1H4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR1H4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR1H4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR1H4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.