Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FUS/TLS Double Nickase Plasmid (m): sc-433326-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FUS/TLS Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FUS/TLS Double Nickase Plasmid (m) and FUS/TLS Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Fus. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FUS/TLS Antibody (4H11): sc-47711
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FUS/TLS Double Nickase Plasmid (m) | sc-433326-NIC | 20 µg | $410.00 | |||
FUS/TLS Double Nickase Plasmid (m2) | sc-433326-NIC-2 | 20 µg | $410.00 |
Fus encodes the RNA-binding protein FUS/TLS, a predominantly nuclear factor that coordinates multiple steps of gene expression, including transcriptional regulation, pre-mRNA splicing, mRNA transport, and stress granule dynamics. FUS/TLS participates in DNA damage responses and genome maintenance through interactions with repair machinery and chromatin-associated complexes, linking RNA processing to cellular homeostasis. Disruption of FUS/TLS function alters neuronal RNA metabolism and proteostasis, and aberrant localization or aggregation has been widely implicated in neurodegeneration, making Fus a key target for mechanistic studies of RNA biology in the nervous system. In mouse models, perturbing Fus provides a tractable system to interrogate RNA regulatory networks and pathways connected to DNA repair, stress responses, and neurodegenerative phenotypes.
FUS/TLS Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Fus locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Fus. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Fus function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Fus-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.