Fura-2, pentasodium salt is a favorable ratiometric calcium indicator. Fura-2 is excitation-ratioable while Indo-1 (sc-491362) is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. Fura-2 sodium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode.
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