FucT-VIII Double Nickase Plasmid (h): sc-402957-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FucT-VIII Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FucT-VIII Double Nickase Plasmid (h) and FucT-VIII Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FUT8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FucT-VIII Antibody (B-10): sc-271244
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FucT-VIII Double Nickase Plasmid (h) | sc-402957-NIC | 20 µg | $410.00 | |||
FucT-VIII Double Nickase Plasmid (h2) | sc-402957-NIC-2 | 20 µg | $410.00 |
Human FUT8 encodes α1,6-fucosyltransferase (FucT-VIII), the Golgi-localized enzyme that catalyzes core fucosylation of N-linked glycans on glycoproteins. This modification is a key determinant of glycoprotein maturation, receptor–ligand interactions, and downstream signal transduction, influencing processes such as cell adhesion, immune receptor function, and growth factor signaling. FUT8 activity integrates with the N-glycosylation pathway and cellular trafficking through the secretory pathway, shaping protein stability and surface presentation. Dysregulated core fucosylation has been associated with altered signaling networks and glycoprotein remodeling observed in multiple disease-relevant contexts, including inflammation and oncogenic transformation.
FucT-VIII Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FUT8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FUT8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FUT8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FUT8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.