frizzled Double Nickase Plasmid (h): sc-401592-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- frizzled-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- frizzled-2 Double Nickase Plasmid (h) and frizzled-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FZD2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
frizzled Double Nickase Plasmid (h) | sc-401592-NIC | 20 µg | $410.00 | |||
frizzled-2 Double Nickase Plasmid (h2) | sc-401592-NIC-2 | 20 µg | $410.00 |
FZD2 encodes the human frizzled-2 receptor, a seven-transmembrane WNT receptor that coordinates ligand-dependent activation of canonical β-catenin signaling and non-canonical planar cell polarity and WNT/Ca²⁺ pathways. Through interactions with WNT ligands and co-receptors, FZD2 modulates cell fate specification, polarity, proliferation, and migratory behavior during development and tissue homeostasis. Altered FZD2 expression or signaling output has been associated with dysregulated WNT pathway activity observed across diverse disease-relevant contexts, including tumor-associated EMT programs and aberrant tissue remodeling. Because WNT–FZD2 signaling can reshape transcriptional networks and cytoskeletal dynamics, FZD2 is frequently studied in pathway mapping, receptor–ligand specificity, and mechanisms controlling invasion and differentiation.
frizzled-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FZD2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FZD2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FZD2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FZD2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.