Date published: 2025-9-5
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FOXP3 Antibody (2A11G9) - Immunofluorescence - Image 15949
FOXP3 抗体 (2A11G9): sc-53876. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞核和细胞质定位.
FOXP3 抗体 (2A11G9): sc-53876. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞核和细胞质定位.

FOXP3 抗体 (2A11G9): sc-53876

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寫評論提問

说明书
  • FOXP3 抗体 2A11G9 是小鼠单克隆 IgG2a κ，FOXP3抗体, 在40篇文献中引用，规格为200 µg/ml
  • 针对纯化的截短重组FoxP3的human
  • FOXP3 抗体 (2A11G9) 推荐用于 IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 FOXP3
  • FOXP3抗体（2A11G9）可与琼脂糖结合用于IP；与HRP结合用于IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于 IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于 IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FOXP3 (2A11G9): sc-53876的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是FOXP3 Antibody (2A11G9) 适用于 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与FOXP3 Antibody (2A11G9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外FOX抗体，包括FOXC1, FOXC2, FOXD1, FOXD3, FOXE3, FOXF2, FOXJ1, FOXJ2, FOXK1, FOXL1, FOXL2, FOXM1, FOXN1, FOXN2, FOXN4和FOXP1

FOXP3 抗体（2A11G9）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人源的 FOXP3 蛋白。FOXP3 (2A11G9) 抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。FOXP3 又称 scurfin，通过作为转录抑制因子在维持免疫平衡方面发挥着关键作用。FOXP3 对调节性 T 细胞的发育和功能至关重要，而调节性 T 细胞对预防自身免疫性疾病和维持对自身抗原的耐受性至关重要。FOXP3 基因突变可导致免疫调节失调，从而引起严重的 X 连锁疾病，即免疫调节失调、多内分泌病、肠病、X 连锁综合征（IPEX）。FOXP3 能够通过其叉头结构域与 DNA 结合，从而调节参与 T 细胞活化和分化的基因，这凸显了 FOXP3 在免疫反应中的重要性。编码 FOXP3 的基因位于 X 染色体的 Xp11.23 位，FOXP3 的表达在 T 细胞发育过程中受到严格调控，因此 FOXP3 是在各种研究和临床应用中识别和研究调节性 T 细胞的重要标志物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FOXP3 抗体 (2A11G9) 参考文献:

  1. 人类 7q31 上的 SPCH1 区域：关键区间的基因组特征以及与言语和语言障碍相关的易位定位。  |  Lai, CS., et al. 2000. Am J Hum Genet. 67: 357-68. PMID: 10880297
  2. 免疫调节紊乱, 多内分泌病, 肠病, X 连锁综合征（IPEX）是由 FOXP3 基因突变引起的。  |  Bennett, CL., et al. 2001. Nat Genet. 27: 20-1. PMID: 11137993
  3. 一种新的叉头/翼螺旋蛋白--scurfin--的破坏导致了scurfy小鼠致命的淋巴组织增生性疾病。  |  Brunkow, ME., et al. 2001. Nat Genet. 27: 68-73. PMID: 11138001
  4. 在肺部表达并作为转录抑制因子的翼螺旋/叉头（Fox）基因新亚科的特征。  |  Shu, W., et al. 2001. J Biol Chem. 276: 27488-97. PMID: 11358962
  5. Scurfin（FOXP3）是一种转录抑制因子，可调节 T 细胞的活化。  |  Schubert, LA., et al. 2001. J Biol Chem. 276: 37672-9. PMID: 11483607
  6. FOXP1翼螺旋转录因子是染色体 3p 上的一个新的候选肿瘤抑制基因。  |  Banham, AH., et al. 2001. Cancer Res. 61: 8820-9. PMID: 11751404
  7. FOXP3及其在免疫系统中的作用。  |  Kim, CH. 2009. Adv Exp Med Biol. 665: 17-29. PMID: 20429413
  8. 两种FOXP3(+)CD4(+) T细胞亚群明显控制了结直肠癌的预后。  |  Saito, T., et al. 2016. Nat Med. 22: 679-84. PMID: 27111280
  9. 人类FOXP3+调节性T细胞在自身免疫和癌症中的异质性和功能。  |  Wing, JB., et al. 2019. Immunity. 50: 302-316. PMID: 30784578
  10. 人类FOXP3和肿瘤微环境。  |  Wang, J., et al. 2023. Immunology. 168: 248-255. PMID: 35689826
  11. 遗传追踪揭示了外周诱导的调节性T细胞中Foxp3依赖性和Foxp3非依赖性的转录因子功能。  |  van der Veeken, J., et al. 2022. Immunity. 55: 1173-1184.e7. PMID: 35700740
  12. FOXP3通过多聚化识别微卫星并连接DNA。  |  Zhang, W., et al. 2023. Nature. 624: 433-441. PMID: 38030726

订购信息

产品名称产品编号规格价格数量收藏夹

FOXP3 抗体 (2A11G9)

sc-53876
200 µg/ml
$316.00

FOXP3 (2A11G9): m-IgG Fc BP-HRP 套装

sc-528584
200 µg Ab; 10 µg BP
$354.00

FOXP3 (2A11G9): m-IgGκ BP-HRP 套装

sc-520996
200 µg Ab, 40 µg BP
$354.00

FOXP3 (2A11G9): m-IgG2a BP-HRP 套装

sc-547125
200 µg Ab; 10 µg BP
$354.00

FOXP3 抗体 (2A11G9) AC

sc-53876 AC
500 µg/ml, 25% agarose
$416.00

FOXP3 抗体 (2A11G9) HRP

sc-53876 HRP
200 µg/ml
$316.00

FOXP3 抗体 (2A11G9) FITC

sc-53876 FITC
200 µg/ml
$330.00

FOXP3 抗体 (2A11G9) PE

sc-53876 PE
200 µg/ml
$343.00

FOXP3 抗体 (2A11G9) Alexa Fluor® 488

sc-53876 AF488
200 µg/ml
$357.00

FOXP3 抗体 (2A11G9) Alexa Fluor® 546

sc-53876 AF546
200 µg/ml
$357.00

FOXP3 抗体 (2A11G9) Alexa Fluor® 594

sc-53876 AF594
200 µg/ml
$357.00

FOXP3 抗体 (2A11G9) Alexa Fluor® 647

sc-53876 AF647
200 µg/ml
$357.00

FOXP3 抗体 (2A11G9) Alexa Fluor® 680

sc-53876 AF680
200 µg/ml
$357.00

FOXP3 抗体 (2A11G9) Alexa Fluor® 790

sc-53876 AF790
200 µg/ml
$357.00

my customer order FOXP3 (2A11G9): sc-53876 conjugated with PE, she need to use it for flow cytometry application , her question what is the solvent should be use for reconstitution and the volume. what will be the vial concentration after reconstitution

Asked by: muneer
Thank you for your question . Please contact technical service by phone or email . (800)-457-3801 or scbt@scbt.com
Answered by: BlakeJ
Date published: 2022-11-14

请问这个抗体用来做细胞免疫荧光效果怎样

Asked by: JINGJINGJING123456789
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended dilution for Foxp3 anti mouse for IHC staining?

Asked by: Maysa
Thank you for your question. We recommend a starting dilution of 1:50 for this antibody for IHC staining. Here is a link to the preferred protocol: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2017-12-05

Why FOXP3 molecular weight is 48KDa and we see the band between 55 and 90KDa? Thank you

Asked by: mayo2n
Thank you for your question. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to provide you further information. The product is recommended for detection of FOXP3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge.
Answered by: Technical Support Europe
Date published: 2021-10-15

Hi, I asked you before about the best ab to detect Foxp3 for human tissue by IHC-P and you advice me by sc53876. I would like to ask about sc-130666 and sc130666 PerCP, whats the different. If I would like to test sc130666 in IHC-P it will possible or not

Asked by: shaikhah
Thank you for your question. sc-130666 PerCP is conjugated to a fluorescent peridinin-chlorophyll protein complex (PerCP) and, therefore, a secondary detection reagent is not needed. Whereas sc-130666 is unconjugated so it would require use of a secondary detection reagent. sc-130666:FOXP3 (150D/E4) is recommended for use in IHC with paraffin embedded sections. For further information regarding collaboration, please contact our Technical Service Department and we would be happy to discuss this with you.
Answered by: Santa Cruz TS
Date published: 2017-03-24

if i would like to detect Foxp3 (mouse momclonal ) of human tissue by using IHC-P. which is the best one?please advice me

Asked by: shaikhah
Thank you for your question. This monoclonal antibody, sc-53876, would be the best choice for IHC(P) use on human tissues, so this is the one that I would recommend. If you have any further questions, please contact our Technical Service team.
Answered by: Tech Service
Date published: 2017-03-24

Which negative control should be used when using the FITC conjugated FOXP3 (2A11G9): sc-53876 in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using isotype specific normal mouse IgG2a conjugated to FITC, sc-2856
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 4 out of 5 by from This antibody works well for IFC and IHQ(P)This antibody works well for immunofluorescence and immunohistochemistry (mouse lung tumor tissue)
Date published: 2023-06-20
Rated 3 out of 5 by from WB testI used this antibody to detect the expression of foxp3 protein in mouse intestinal tissue, but there are at least two molecular weight bands on the pvdf membrane. Which is the specific band?
Date published: 2021-10-15
Rated 5 out of 5 by from Great antibodyThis antibody is very good for western blot ,strong signal.
Date published: 2019-02-13
Rated 5 out of 5 by from Great antibody for WBI received a small sample and tried it for detection of FOXP3 in skeletal muscle samples (rat). It worked well in Western blots at 1:500 concentration, producing a strong signal at predicted MW.
Date published: 2018-03-08
Rated 1 out of 5 by from Several weak bandsI used this Ab on mouse lung homogenates at 1:1000 dilution. There were multiple bands, high background, and the bands were not strong. The datasheet says the band will be at 48kda, but the image shows it well about 55kda... I had two weak bands around that area.
Date published: 2018-03-01
Rated 5 out of 5 by from Quite good antibody1:500 diluted for wb of rat protein ,the band is strong and special
Date published: 2017-12-30
Rated 5 out of 5 by from great in WB & IHCtissue: spleen&liver WB, 1:1000 diluted in 5%BSA(TBST), incubate 4℃ overnight, and IgG-HRP, RT, 1hour. IHC- Aorta root 1:50 diluted is great
Date published: 2017-09-17
Rated 5 out of 5 by from 很好的抗体好的很，效果不错的。这的荧光效果非常的好. 并且是单克隆 抗体，建议大家 购买此货号的抗体来作免疫荧光实验的数据
Date published: 2017-07-28
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FOXP3 抗体 (2A11G9) is rated 4.1 out of 5 by 21.
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