Fluorescein diacetate stains living cells green, and is a substrate for esterases. Fluorescein Diacetate is suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
Clements, K.M., et al. 2001. Osteoarthritis Cartilage 9, 499. Bedner, E., et al. 1998. Cytometry 33, 1. Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.
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Various publications cite the use of FluoresceinVarious publications cite the use of Fluorescein Diacetate in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes. -SCBT Publication Review
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