Fluorescein Diacetate CAS: 596-09-8
MF: C24H16O7
MW: 416.38

Fluorescein Diacetate (CAS 596-09-8)

Fluorescein Diacetate | CAS 596-09-8 is rated 5.0 out of 5 by 1.
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Alternate Names: Fluorescein diacetate is also known as Cellstain-FDA, Di-O-acetylfluorescein, and 3,6-Diacetoxyfluoran
Application: Fluorescein Diacetate is a useful live-cell fluorescent stain that is hydrolysed to fluorescent fluorescein in live cells
CAS Number: 596-09-8
Purity: ≥97%
Molecular Weight: 416.38
Molecular Formula: C24H16O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Fluorescein diacetate stains living cells green, and is a substrate for esterases. Fluorescein Diacetate is suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.


References

Clements, K.M., et al. 2001. Osteoarthritis Cartilage 9, 499. Bedner, E., et al. 1998. Cytometry 33, 1. Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in chloroform, DMSO, ethanol, and water.
Storage :
Store at -20° C
Melting Point :
200-203° C
Boiling Point :
~604.70° C at 760 mmHg (Predicted)
Density :
~1.47 g/cm3 (Predicted)
Refractive Index :
n20D 1.68 (Predicted)
IC50 :
HEK293 : IC50 = >89.2 µM (human); Beta Lactamase: IC50 = 39.8 µM (Pseudomonas aeruginosa); HIV-1(RF): IC50 = 43.4 µM (CEM); HIV-1(RF): EC5050 = >200 µM (CEM); HEK293 : EC5050 = >54.7 µM (human); Insulin-degrading enzyme: EC5050 = >54.7 µM (human)
Fluoresence :
λex 495 nm, λem 520 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
65047
MDL Number :
MFCD00005062
EC Number :
209-877-6
Beilstein Registry :
62575
SMILES :
CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC=CC=C5C(=O)O3

Download SDS (MSDS)

Certificate of Analysis

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Fluorescein Diacetate (CAS 596-09-8) Product Citations

See how others have used Fluorescein Diacetate (CAS 596-09-8). Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 27000946  Pandurangan, M. et al. 2016. J. Mol. Recognit. 29: 426-35.

PMID: # 22345351  Poloz, Y. et al. 2012. Eukaryotic Cell. 11: 545-557.

PMID: # 30298620  Brain Behav. 8: e01037.

PMID: # 30500539  Cell. 175: 1607-1619.e15.

Citations 1 to 4 of 4 total

Dear Sir / Ma'am, plz tell me cost of your product Fluoresceine diacetate.

Asked by: Shubham
Thank you for your question. Please contact one of our authorized distributors for information about current product availability and shipping lead times.
Answered by: Technical Support
Date published: 2019-02-13
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Rated 5 out of 5 by from Various publications cite the use of Fluorescein Various publications cite the use of Fluorescein Diacetate in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes. -SCBT Publication Review
Date published: 2015-05-07
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