Fluorescein Antibody (6A4): sc-57585

Fluorescein Antibody (6A4) is a mouse monoclonal IgM antibody that detects Fluorescein protein of non-species reactive by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Fluorescein antibody (6A4) is available as a non-conjugated format, providing researchers with a versatile tool for various applications. Fluorescein, a well-known fluorophore, plays a crucial role in numerous scientific fields due to unique optical properties, including an absorption maximum at 490 nm and an emission maximum at 514 nm. Fluorescein′s structure, characterized by xanthene ring system, is essential for fluorescent capabilities, allowing effective utilization in microscopy and environmental testing. Fluorescein′s ability to emit bright fluorescence upon excitation makes Fluorescein invaluable in tracking water leaks in environmental studies, as well as serving as a diagnostic agent in ophthalmology, where topical application aids in identifying corneal abrasions, ulcers, herpetic infections, and dry eye conditions. With a molecular mass of 332.32 g/mol, Fluorescein can be synthesized through various methods, including reaction of phthalic anhydride and resorcinol in presence of zinc chloride or using methanesulfonic acid as a catalyst, further highlighting significance in both research and clinical applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fluorescein Antibody (6A4) References:

1. Sodium fluorescein-induced changes in leukocyte-endothelial interaction in an animal model.  |  Sobral do Rosário, H., et al. 2000. Microvasc Res. 59: 386-9. PMID: 10792970
2. Treatment of ocular methylrosaniline poisoning with fluorescein solution.  |  HOSFORD, GN. and SMITH, JG. 1952. J Am Med Assoc. 150: 1482-4. PMID: 12990455
3. Ultrabright fluorescein-labeled antibodies near silver metallic surfaces.  |  Lakowicz, JR., et al. 2004. Biopolymers. 74: 467-75. PMID: 15274090
4. Fluorescence-based detection of thiols in vitro and in vivo using dithiol probes.  |  Pullela, PK., et al. 2006. Anal Biochem. 352: 265-73. PMID: 16527239
5. Lid wiper epitheliopathy and dry eye symptoms.  |  Hinkle, DM. 2006. Eye Contact Lens. 32: 160; author reply 160. PMID: 16702876
6. Fluorophotometry as a diagnostic tool for the evaluation of dry eye disease.  |  Fahim, MM., et al. 2006. BMC Ophthalmol. 6: 20. PMID: 16729882
7. Topical intranasal fluorescein: the missing partner in algorithms of cerebrospinal fluid fistula detection.  |  Saafan, ME., et al. 2006. Laryngoscope. 116: 1158-61. PMID: 16826052
8. Comparative evaluation of oral fluorescein angiography using the confocal scanning laser ophthalmoscope and digital fundus camera with intravenous fluorescein angiography using the digital fundus camera.  |  Azad, RV., et al. 2006. Clin Exp Ophthalmol. 34: 425-9. PMID: 16872337
9. Optimization of anterior eye fluorescein viewing.  |  Peterson, RC., et al. 2006. Am J Ophthalmol. 142: 572-5. PMID: 17011847

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fluorescein Antibody (6A4) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fluorescein Antibody (6A4) Specifications

Alternate Product Name: Fluorescein

Product Clone Name: 6A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM

Amino Acids:

Epitope Target: Fluorescein

Epitope Species:

Product Reactivity/Detection: free and bound fluorescein

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 50 µg IgM in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Fluorescein Antibody (6A4): N/A