Fluorescein 5(6)-isothiocyanate CAS: 27072-45-3
MF: C21H11NO5S
MW: 389.38
A useful fluorescent labeling reagent for proteins.

Fluorescein 5(6)-isothiocyanate (CAS 27072-45-3)

Fluorescein 5(6)-isothiocyanate | CAS 27072-45-3 is rated 5.0 out of 5 by 1.
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Alternate Names: FITC; Fluorescein isothiocyanate
Application: Fluorescein 5(6)-isothiocyanate is a useful fluorescent labeling reagent for proteins
CAS Number: 27072-45-3
Molecular Weight: 389.38
Molecular Formula: C21H11NO5S
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Fluorescein 5(6)-isothiocyanate (FITC) is an isothiocyanate-derivitized Fluorescein (sc-215040, sc-206026) fluorescent dye compound used for immunofluorescent identification and immunofluorescent histology applications. Fluorescein 5(6)-isothiocyanate reacts with nucleophilic amine and thiol amino acid residues to covalently link the compound to the protein of investigation. Fluorescein isothiocyanates have been described for use in the immunofluorescent identification of microorganisms and for the immunohistochemical localization of proteins.


References

1. Riggs, J.L., et al. 1958. Amer. J. Pathol., 34: 1081-1097. PMID: 13583098
2. Sweet, G.H. and Schindler, C.A. 1967. J. Bacteriol. 93: 1943-1948. PMID: 5337774
3. Geffen, L.B., et al. 1969. J. Physiol. 204: 593-605. PMID: 4980932
2. Mann, K.G. and Fish, W.W. 1972. Methods Enzymol. 26: 28-42. PMID: 4680709
5. Sakal, E., et al. 1991. Biochemistry. 30: 8899-8904. PMID: 1909577

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (5 mg/ml), methanol, DMF, and ethanol (20 mg/ml). Insoluble in water.
Storage :
Store at 4° C
Melting Point :
>255° C (dec.)
Boiling Point :
~708.6° C at 760 mmHg (Predicted)
Density :
~1.5 g/cm3 (Predicted)
Refractive Index :
n20D 1.75 (Predicted)
IC50 :
L1210: IC50 = 700 µM (mouse)
pK Values :
pKa: 9.31 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
18730
Merck Index :
14: 4089
MDL Number :
MFCD00005063
EC Number :
248-207-7
Beilstein Registry :
1407295
SMILES :
C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O

Download SDS (MSDS)

Certificate of Analysis

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Fluorescein 5(6)-isothiocyanate (CAS 27072-45-3) Product Citations

See how others have used Fluorescein 5(6)-isothiocyanate (CAS 27072-45-3). Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 22212092  Zhang, Y. et al. 2012. J. Thromb. Haemost. 10: 411-418.

Citations 1 to 1 of 1 total

Hello, I would like to order fluorescein isothiocyanate (27072-45-3). Do you have it in your stock. If, yes could you send me a quote. Thank you. Best regards. Killian Oukoloff

Asked by: Killian
Thank you for your question. Please contact our Customer Care Department to inquire about current product availability and to request a quote. Please call us toll-free at (800) 457-3801 option 1, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-29
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Rated 5 out of 5 by from Olszowski T; et al Olszowski T; et al. (PubMed ID: 26370970) utilized Fluorescein 5(6)-isothiocyanate to measure the extent of apoptosis of THP-1 derived macrophages, in flow cytometry. -SCBT Publication Review
Date published: 2015-02-14
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