Fluorescein 5(6)-isothiocyanate (FITC) is an isothiocyanate-derivitized Fluorescein (sc-215040, sc-206026) fluorescent dye compound used for immunofluorescent identification and immunofluorescent histology applications. Fluorescein 5(6)-isothiocyanate reacts with nucleophilic amine and thiol amino acid residues to covalently link the compound to the protein of investigation. Fluorescein isothiocyanates have been described for use in the immunofluorescent identification of microorganisms and for the immunohistochemical localization of proteins.
1. Riggs, J.L., et al. 1958. Amer. J. Pathol., 34: 1081-1097. PMID: 13583098 2. Sweet, G.H. and Schindler, C.A. 1967. J. Bacteriol. 93: 1943-1948. PMID: 5337774 3. Geffen, L.B., et al. 1969. J. Physiol. 204: 593-605. PMID: 4980932 2. Mann, K.G. and Fish, W.W. 1972. Methods Enzymol. 26: 28-42. PMID: 4680709 5. Sakal, E., et al. 1991. Biochemistry. 30: 8899-8904. PMID: 1909577
Soluble in DMSO (5 mg/ml), methanol, DMF, and ethanol (20 mg/ml). Insoluble in water.
Store at 4° C
>255° C (dec.)
~708.6° C at 760 mmHg (Predicted)
~1.5 g/cm3 (Predicted)
n20D 1.75 (Predicted)
L1210: IC50 = 700 µM (mouse)
pKa: 9.31 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I would like to order fluorescein isothiocyanate (27072-45-3).
Do you have it in your stock.
If, yes could you send me a quote.
Thank you. Best regards. Killian Oukoloff
Asked by: Killian
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Rated 5 out of
Olszowski T; et alOlszowski T; et al. (PubMed ID: 26370970) utilized Fluorescein 5(6)-isothiocyanate to measure the extent of apoptosis of THP-1 derived macrophages, in flow cytometry. -SCBT Publication Review
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