FLUO 3/AM, a visible wavelength calcium probe, can be detected through fluorescent cytometry. Experiments show that this compound can be loaded into lymphocytes and fibroblasts by incubation with the pentaacetoxymethyl ester of the dye. Once inside the cell, the ester is hydrolyzed to yield genuine FLUO-3/AM capable of indicating changes in Ca2+. Other studies have reported that this FLUO 3/AM monitors Ca2+ levels without interfering with intracellular conditions. Additionally, this agent has been noted to be an effective probe for assessing the concentration and kinetics of change in mitochondrial Ca2+ under variety of conditions.
1. Kao, J.P., et al. 1989. J. Biol. Chem. 264: 8179-8184. PMID: 2498309
2. Saavedra-Molina, A., et al. 1990. Biochem. Biophys. Res. Commun. 167: 148-153. PMID: 2310386
3. Schumann, M.A., et al. 1993. J. Biol. Chem. 268: 2134-2140. PMID: 8380590
4. Caputo, C. and Bolaños, P. 1994. J. Physiol. 481: 119-128. PMID: 7853234
See how others have used FLUO 3/AM (CAS 121714-22-5). Click on the entry to view the PubMed entry .
PMID: # 31143124 Trachtenberg, A.|Muduli, S.|Sidoryk, K.|Cybulski, M.|Danilenko, M.| et al. 2019. Front Pharmacol. 10: 507.
PMID: # 25972311 Lee, PY. et al. 2015. The Journal of surgical research. 197: 390-7.
PMID: # 26632252 Singh, R. et al. 2015. Sci Rep. 5: 17454.
PMID: # 24520246 Liu, G. et al. 2014. Exp Ther Med. 7: 565-572.
PMID: # 22262690 Zhang, WS. et al. 2012. Biology of reproduction. 86: 154, 1-7.
PMID: # 22288713 Sun, R. et al. 2012. Cell Biol. Int. 36: 475-81.
PMID: # 21593605 Chhabra, R. et al. RNA biology. 8: 648-64.
Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.