Floxuridine-13C,15N2

Floxuridine-13C,15N2 is a stable isotope-labeled variant of floxuridine, where carbon and nitrogen atoms are replaced by their isotopes, carbon-13 (13C) and nitrogen-15 (15N). This specific labeling enhances the utility of the compound in scientific research, particularly for detailed analytical studies using methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. Floxuridine itself is a nucleoside analog, specifically a thymidine analog, which integrates into DNA, disrupting the normal process of DNA synthesis. The primary mechanism of action for floxuridine involves the inhibition of thymidylate synthase, an enzyme that catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). This process is crucial for DNA replication and repair. By inhibiting this enzyme, floxuridine prevents the production of thymidine triphosphate (dTTP), one of the four nucleotides required for DNA synthesis, leading to the incorporation of erroneous nucleotides into DNA and subsequent DNA strand breaks or apoptosis. In research, Floxuridine-13C,15N2 is used to trace the metabolic pathways and DNA incorporation dynamics of floxuridine more accurately, as the isotopic labels allow for precise tracking and quantification of the molecule in various biological matrices. This capability is vital for studies aimed at understanding the metabolism and biological effects of floxuridine at the molecular level. Additionally, Floxuridine-13C,15N2 is utilized in studies examining the mechanisms of action of nucleoside analogs in DNA synthesis. Researchers use this labeled compound to investigate how modifications to nucleoside structure influence their incorporation into DNA and the subsequent biological outcomes, such as cellular response to DNA damage and the activation of repair mechanisms.