Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Flotillin-1 Double Nickase Plasmid (h): sc-400736-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Flotillin-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Flotillin-1 Double Nickase Plasmid (h) and Flotillin-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FLOT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Flotillin-1 Antibody (C-2): sc-74566
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Flotillin-1 Double Nickase Plasmid (h) | sc-400736-NIC | 20 µg | $410.00 | |||
Flotillin-1 Double Nickase Plasmid (h2) | sc-400736-NIC-2 | 20 µg | $410.00 |
FLOT1 encodes flotillin-1, a lipid raft–associated scaffolding protein enriched at the plasma membrane and endosomal compartments, where it helps organize membrane microdomains and coordinate vesicular trafficking. Flotillin-1 participates in clathrin-independent endocytosis, receptor internalization, and cytoskeletal dynamics, influencing signal transduction pathways linked to growth factor and immune receptor signaling. Through its roles in membrane organization and endosomal sorting, FLOT1 can modulate cellular migration, adhesion, and stress responses. Dysregulated flotillin-1 expression or localization has been associated with altered signaling networks and phenotypes relevant to cancer biology and neurodegenerative and inflammatory processes, supporting its use as a mechanistic target in cell signaling and trafficking studies.
Flotillin-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FLOT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FLOT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FLOT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FLOT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.