Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Flightless I Double Nickase Plasmid (h): sc-401667-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Flightless I Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Flightless I Double Nickase Plasmid (h) and Flightless I Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FLII. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Flightless I Antibody (116.40): sc-21716
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Flightless I Double Nickase Plasmid (h) | sc-401667-NIC | 20 µg | $410.00 | |||
Flightless I Double Nickase Plasmid (h2) | sc-401667-NIC-2 | 20 µg | $410.00 |
FLII encodes Flightless I, an evolutionarily conserved gelsolin family actin-remodeling protein that coordinates cytoskeletal dynamics with transcriptional regulation. Flightless I participates in cell migration, adhesion, and wound-response programs through modulation of actin architecture and interactions with signaling networks such as Rho GTPase-dependent pathways and focal adhesion–associated processes. In the nucleus, it can influence chromatin-associated complexes and transcriptional outputs that shape inflammatory and stress-responsive gene expression. Dysregulated FLII activity has been linked in the literature to altered epithelial repair, immune signaling phenotypes, and tumor-associated cell motility, making it a useful target for mechanistic studies of cytoskeleton-to-nucleus communication.
Flightless I Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FLII locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FLII. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FLII function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FLII-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.