Fli-1 Antibody (F-12): sc-365294

Fli-1 Antibody (F-12) is a mouse monoclonal IgM antibody that detects Fli-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Fli-1 antibody (F-12) is available as the non-conjugated form. Fli-1 plays a crucial role in regulating gene expression during hematopoiesis and is involved in the development of various tissues, including the cardiovascular system. Fli-1 acts as a transcription factor that binds to specific DNA sequences, thereby influencing the expression of genes necessary for cell differentiation and proliferation. Dysregulation of Fli-1 has been implicated in several diseases, including certain types of cancer, making Fli-1 a significant target for research and therapeutic interventions. Fli-1 is part of the Ets family of transcription factors, which are characterized by a conserved DNA-binding domain that allows interaction with specific motifs in the promoter regions of target genes. Understanding Fli-1′s role in cellular processes not only sheds light on normal developmental biology but also provides insights into pathological conditions where Fli-1 function may be altered.

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Fli-1 Antibody (F-12) References:

1. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells.  |  Xin, JH., et al. 1992. Genes Dev. 6: 481-96. PMID: 1547944
2. Ewing's sarcoma fusion protein, EWS/Fli-1 and Fli-1 protein induce PLD2 but not PLD1 gene expression by binding to an ETS domain of 5' promoter.  |  Kikuchi, R., et al. 2007. Oncogene. 26: 1802-10. PMID: 16964281
3. Caveolin-1 (CAV1) is a target of EWS/FLI-1 and a key determinant of the oncogenic phenotype and tumorigenicity of Ewing's sarcoma cells.  |  Tirado, OM., et al. 2006. Cancer Res. 66: 9937-47. PMID: 17047056
4. The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins.  |  Burtis, KC., et al. 1990. Cell. 61: 85-99. PMID: 2107982
5. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints.  |  Rao, VN., et al. 1989. Science. 244: 66-70. PMID: 2539641
6. Fli-1 overexpression in erythroleukemic cells promotes erythroid de-differentiation while Spi-1/PU.1 exerts the opposite effect.  |  Vecchiarelli-Federico, LM., et al. 2017. Int J Oncol. 51: 456-466. PMID: 28586009
7. Fli-1 Governs Pericyte Dysfunction in a Murine Model of Sepsis.  |  Li, P., et al. 2018. J Infect Dis. 218: 1995-2005. PMID: 30053030
8. Identification and preferential expression in thymic and bursal lymphocytes of a c-ets oncogene-encoded Mr 54,000 cytoplasmic protein.  |  Ghysdael, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 1714-8. PMID: 3006066
9. erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation.  |  Rao, VN., et al. 1987. Science. 237: 635-9. PMID: 3299708
10. Identification of Mir-182-3p/FLI-1 Axis as a Key Signaling in Immune Response in Cervical Cancer: A Comprehensive Bioinformatic Analysis.  |  Salmerón-Bárcenas, EG., et al. 2023. Int J Mol Sci. 24: PMID: 37047006
11. The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
12. Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation.  |  Pongubala, JM., et al. 1993. Science. 259: 1622-5. PMID: 8456286

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fli-1 Antibody (F-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Fli-1 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fli-1 Antibody (F-12) Specifications

Alternate Product Name: Fli-1

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 427-452

Epitope Target: Fli-1

Epitope Species: mouse

Product Reactivity/Detection: Fli-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Fli-1 Antibody (F-12): P: Blocking Peptide, X: TransCruz Reagent