Molecular structure of FlAsH-EDT2, CAS Number: 212118-77-9
FlAsH-EDT2 (CAS 212118-77-9)
See product citations (5)
Alternate Names:
FlAsH-EDT2 also known as 4,5-Bis(1,3,2-dithiarsolan-2-yl)-3,6-dihydroxyspiro[isobenzofuran-1(3H),9-[9H]xanthen]-3-one; FlAsH; Fluorescein Arsenical Helix Binder; Lumio Green
Application:
FlAsH-EDT2 is a membrane permeant fluorogenic biarsenical fluorescence probe for labeling proteins
CAS Number:
212118-77-9
Molecular Weight:
664.50
Molecular Formula:
C24H18As2O5S4
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
FlAsH-EDT2 is a fluorescence probe for labeling proteins. The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin. FlAsH-EDT2 binds covalently to a tetracysteine sequence (CCPGCC).
FlAsH-EDT2 (CAS 212118-77-9) References
- Fluorescent labeling of recombinant proteins in living cells with FlAsH. | Griffin, BA., et al. 2000. Methods Enzymol. 327: 565-78. PMID: 11045009
- The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins. | Stroffekova, K., et al. 2001. Pflugers Arch. 442: 859-66. PMID: 11680618
- Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling. | Ignatova, Z. and Gierasch, LM. 2004. Proc Natl Acad Sci U S A. 101: 523-8. PMID: 14701904
- A new protein conformation indicator based on biarsenical fluorescein with an extended benzoic acid moiety. | Nakanishi, J., et al. 2004. Anal Sci. 20: 273-8. PMID: 15055950
- Recent advances in fluorescent labeling techniques for fluorescence microscopy. | Suzuki, T., et al. 2007. Acta Histochem Cytochem. 40: 131-7. PMID: 18224244
- Concerted but noncooperative activation of nucleotide and actuator domains of the Ca-ATPase upon calcium binding. | Chen, B., et al. 2008. Biochemistry. 47: 12448-56. PMID: 18956892
- Bisarsenical labeling of HIV-1 for real-time fluorescence microscopy. | Arhel, NJ. and Charneau, P. 2009. Methods Mol Biol. 485: 151-9. PMID: 19020824
- Nrf2 responses and the therapeutic selectivity of electrophilic compounds in chronic lymphocytic leukemia. | Wu, RP., et al. 2010. Proc Natl Acad Sci U S A. 107: 7479-84. PMID: 20368435
- Visualization of the Npro protein in living cells using biarsenically labeling tetracysteine-tagged classical swine fever virus. | Li, Y., et al. 2014. Virus Res. 189: 67-74. PMID: 24815879
- Characterization of a Replicating Mammalian Orthoreovirus with Tetracysteine-Tagged μNS for Live-Cell Visualization of Viral Factories. | Bussiere, LD., et al. 2017. J Virol. 91: PMID: 28878073
- Detection of oligonucleotides by PNA-peptide conjugates recognizing the biarsenical fluorescein complex FlAsH-EDT2. | Piras, L., et al. 2017. Biochem Biophys Res Commun. 493: 126-131. PMID: 28919425
- Protein Ligation and Labeling Enabled by a C-Terminal Tetracysteine Tag. | Mo, Z., et al. 2022. Angew Chem Int Ed Engl. 61: e202115377. PMID: 35060269
- Direct quantification of cytosolic delivery of drug nanocarriers using FlAsH-EDT2. | Rotem, R., et al. 2023. Nanomedicine. 47: 102626. PMID: 36356708
- Specific covalent labeling of recombinant protein molecules inside live cells. | Griffin, BA., et al. 1998. Science. 281: 269-72. PMID: 9657724
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FlAsH-EDT2, 25 mg | sc-363644 | 25 mg | $430.00 | |||
FlAsH-EDT2, 100 mg | sc-363644A | 100 mg | $1469.00 | |||
FlAsH-EDT2, 1 g | sc-363644B | 1 g | $9180.00 |