FKHR/FOXO1 Antibody (A-6): sc-514610

FKHR Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect FKHR protein, also known as Forkhead in Rhabdomyosarcoma and FOXO1A, a pivotal member of the forkhead box O (FOXO) family of transcription factors. Anti-FKHR antibody (A-6) is raised against amino acids 471-600 near the C-terminus of human FKHR, ensuring effective recognition of FKHR in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FKHR/FOXO1A is a ubiquitously expressed protein that dynamically shuttles between the cytoplasm and nucleus, a process regulated by phosphorylation via the serine/threonine kinase Akt1. This translocation is crucial for FKHR′s role in regulating gene expression related to apoptosis, cell cycle control, and oxidative stress resistance, thereby maintaining cellular homeostasis and preventing uncontrolled cell proliferation. Under conditions of cellular stress or apoptotic signals, FKHR translocates to the nucleus to activate specific target genes, highlighting an essential role in cellular responses and cancer biology. Additionally, genetic mutations and translocations involving FKHR, such as those found in alveolar rhabdomyosarcomas, result in fusion proteins that act as oncogenic transcription factors, further emphasizing the importance of accurate detection and study. FKHR monoclonal antibody (A-6) serves as an invaluable tool for researchers investigating the mechanisms of FOXO1A-mediated transcriptional regulation and its implications in health and disease.

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FKHR/FOXO1 Antibody (A-6) References:

1. Detection of PAX3-FKHR and PAX7-FKHR fusion transcripts in rhabdomyosarcoma by reverse transcriptase-polymerase chain reaction using paraffin-embedded tissue.  |  Chen, BF., et al. 1999. Zhonghua Yi Xue Za Zhi (Taipei). 62: 86-91. PMID: 10063718
2. Negative regulation of the forkhead transcription factor FKHR by Akt.  |  Tang, ED., et al. 1999. J Biol Chem. 274: 16741-6. PMID: 10358014
3. FKHR binds the insulin response element in the insulin-like growth factor binding protein-1 promoter.  |  Durham, SK., et al. 1999. Endocrinology. 140: 3140-6. PMID: 10385407
4. Role of three FKHR phosphorylation sites in insulin inhibition of FKHR action in hepatocytes.  |  Scheimann, AO., et al. 2001. Horm Metab Res. 33: 631-8. PMID: 11733864
5. FKHR (FOXO1a) is required for myotube fusion of primary mouse myoblasts.  |  Bois, PR. and Grosveld, GC. 2003. EMBO J. 22: 1147-57. PMID: 12606579
6. Insulin-induced phosphorylation of FKHR (Foxo1) targets to proteasomal degradation.  |  Matsuzaki, H., et al. 2003. Proc Natl Acad Sci U S A. 100: 11285-90. PMID: 13679577
7. Abnormal angiogenesis in Foxo1 (Fkhr)-deficient mice.  |  Furuyama, T., et al. 2004. J Biol Chem. 279: 34741-9. PMID: 15184386
8. Alveolar rhabdomyosarcoma-associated proteins PAX3/FOXO1A and PAX7/FOXO1A suppress the transcriptional activity of MyoD-target genes in muscle stem cells.  |  Calhabeu, F., et al. 2013. Oncogene. 32: 651-62. PMID: 22710712
9. FOXO1A promotes neuropeptide FF transcription subsequently regulating the expression of feeding-related genes in spotted sea bass (Lateolabrax maculatus).  |  Li, Q., et al. 2020. Mol Cell Endocrinol. 517: 110871. PMID: 32450284
10. Structural characterization of the FKHR gene and its rearrangement in alveolar rhabdomyosarcoma.  |  Davis, RJ., et al. 1995. Hum Mol Genet. 4: 2355-62. PMID: 8634710

The preferred FKHR/FOXO1 antibody is FKHR/FOXO1 Antibody (C-9): sc-374427.