Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FKBP51 Double Nickase Plasmid (m): sc-420365-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FKBP51 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FKBP51 Double Nickase Plasmid (m) and FKBP51 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Fkbp5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FKBP51 Antibody (D-4): sc-271547
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP51 Double Nickase Plasmid (m) | sc-420365-NIC | 20 µg | $410.00 | |||
FKBP51 Double Nickase Plasmid (m2) | sc-420365-NIC-2 | 20 µg | $410.00 |
Fkbp5 encodes FKBP51, an immunophilin co-chaperone that binds HSP90 and modulates steroid receptor complexes, including glucocorticoid and progesterone receptors, thereby shaping receptor sensitivity and feedback regulation. FKBP51 also interfaces with stress-response signaling by influencing AKT/PHLPP activity and NF-κB-related inflammatory programs, linking proteostasis with transcriptional control. In mouse systems, altered Fkbp5 expression has been associated with dysregulated hypothalamic–pituitary–adrenal axis signaling, metabolic homeostasis, and neuroimmune processes. These functions make FKBP51 a useful node for dissecting pathways that couple chaperone machinery to endocrine and stress-adaptive phenotypes in vivo and in cultured cells.
FKBP51 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Fkbp5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Fkbp5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Fkbp5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Fkbp5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.