Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FIP1L1 CRISPR/Cas9 KO Plasmid (h): sc-413438
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FIP1L1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FIP1L1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FIP1L1 Antibody (C-10): sc-398392
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FIP1L1 CRISPR/Cas9 KO Plasmid (h) | sc-413438 | 20 µg | $397.00 |
Overview
FIP1L1 encodes a core subunit of the cleavage and polyadenylation specificity factor (CPSF) complex that helps define 3′ end processing sites on pre-mRNAs and coordinates poly(A) tail addition. Through interactions with polyadenylation factors and RNA polymerase II–coupled processing machinery, FIP1L1 contributes to transcript maturation, stability, and regulated gene expression programs. Altered FIP1L1 function is relevant to RNA processing dysregulation and has been implicated in hematologic malignancy biology, notably via the FIP1L1–PDGFRA rearrangement observed in certain myeloid neoplasms. As a result, FIP1L1 is frequently studied in pathways linking mRNA 3′ end formation to cell proliferation, differentiation, and stress-responsive transcriptional outputs.
FIP1L1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FIP1L1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FIP1L1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FIP1L1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FIP1L1 protein expression.
This CRISPR knockout system enables efficient generation of FIP1L1-deficient cell models for investigation of FIP1L1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FIP1L1 exon(s) critical for FIP1L1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FIP1L1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FIP1L1 CRISPR/Cas9 KO Plasmid (h) and FIP1L1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FIP1L1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FIP1L1 HDR Plasmid (h) and FIP1L1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FIP1L1 homology arms to support homology-directed repair at defined FIP1L1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.