Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fibronectin Double Nickase Plasmid (h): sc-400082-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fibronectin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fibronectin Double Nickase Plasmid (h) and Fibronectin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fibronectin Antibody (EP5): sc-8422
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibronectin Double Nickase Plasmid (h) | sc-400082-NIC | 20 µg | $410.00 | |||
Fibronectin Double Nickase Plasmid (h2) | sc-400082-NIC-2 | 20 µg | $410.00 |
FN1 encodes fibronectin, a high-molecular-weight extracellular matrix glycoprotein that assembles into fibrillar networks and provides integrin-binding sites to coordinate cell adhesion, spreading, and migration. Fibronectin regulates focal adhesion and cytoskeletal dynamics and interfaces with ECM remodeling pathways involving integrins, FAK/Src signaling, and Rho-family GTPases, thereby shaping mechanotransduction and tissue architecture. Through its roles in wound repair, angiogenesis, and epithelial–mesenchymal interactions, FN1 is frequently studied in contexts of fibrosis, tumor-associated stroma, and inflammatory tissue remodeling. Altered fibronectin deposition and splice isoform balance are associated with changes in cell invasion, immune cell trafficking, and organ-specific matrix stiffness across multiple disease models.
Fibronectin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.