Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fibrillarin Double Nickase Plasmid (h): sc-400741-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fibrillarin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Fibrillarin Double Nickase Plasmid (h) and Fibrillarin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FBL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fibrillarin Antibody (G-8): sc-374022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibrillarin Double Nickase Plasmid (h) | sc-400741-NIC | 20 µg | $410.00 | |||
Fibrillarin Double Nickase Plasmid (h2) | sc-400741-NIC-2 | 20 µg | $410.00 |
FBL encodes fibrillarin, a conserved nucleolar methyltransferase that catalyzes 2′-O-ribose methylation of rRNA within box C/D snoRNP complexes. This activity is integral to pre-rRNA processing, ribosome biogenesis, and maintenance of nucleolar structure, linking FBL function to global control of protein synthesis and cell growth programs. Fibrillarin also participates in coordination of RNA modification pathways that couple transcription, rRNA maturation, and translation. Altered nucleolar activity and dysregulated ribosome biogenesis involving FBL have been associated with proliferative stress states and are frequently examined in cancer biology and ribosomopathy-focused research.
Fibrillarin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FBL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FBL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FBL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FBL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.