FHL-5 Antibody (F-26): sc-101045

FHL-5 Antibody (F-26) is a mouse monoclonal IgG2a kappa light chain antibody that detects FHL-5 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FHL-5 antibody (F-26) is available as the non-conjugated form. The four-and-a-half-LIM domain (FHL) proteins, which include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2), FHL-4, and FHL-5, are characterized by a unique "half-domain" that consists of a single zinc finger domain located in the N-terminal region, setting them apart from other LIM-only proteins that possess multiple zinc fingers. FHL-5, also known as ACT (Activator of CREM in Testis), plays a crucial role in spermatogenesis as FHL-5 interacts with the transcription factor CREM, which is essential for spermatid differentiation. This interaction occurs through FHL-5′s third LIM domain and enhances CREM activity independently of phosphorylation, indicating that FHL-5 functions as a transcriptional coactivator. Notably, during spermatid elongation, FHL-5 is translocated from the nucleus to the cytoplasm by the kinesin motor protein KIF17, leading to the silencing of CREM activity. This dynamic regulation underscores the importance of FHL-5 in the intricate process of spermatogenesis, making FHL-5 a significant target for research in reproductive biology.

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FHL-5 Antibody (F-26) References:

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2. The structure of the human LIM protein ACT gene and its expression in tumor cell lines.  |  Morgan, MJ. and Whawell, SA. 2000. Biochem Biophys Res Commun. 273: 776-83. PMID: 10873680
3. Cloning and expression of activator of CREM in testis in human testicular tissue.  |  Palermo, I., et al. 2001. Biochem Biophys Res Commun. 283: 406-11. PMID: 11327716
4. A specific programme of gene transcription in male germ cells.  |  Kimmins, S., et al. 2004. Reprod Biomed Online. 8: 496-500. PMID: 15151709
5. Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene.  |  Kotaja, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 10620-5. PMID: 15247423
6. FHL5, a novel actin-binding protein, is highly expressed in eel gill pillar cells and responds to wall tension.  |  Mistry, AC., et al. 2004. Am J Physiol Regul Integr Comp Physiol. 287: R1141-54. PMID: 15284080
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9. Familial hemophagocytic lymphohistiocytosis type 5 (FHL-5) is caused by mutations in Munc18-2 and impaired binding to syntaxin 11.  |  zur Stadt, U., et al. 2009. Am J Hum Genet. 85: 482-92. PMID: 19804848
10. Platelet secretion defect in patients with familial hemophagocytic lymphohistiocytosis type 5 (FHL-5).  |  Sandrock, K., et al. 2010. Blood. 116: 6148-50. PMID: 21183701
11. Defects in neutrophil granule mobilization and bactericidal activity in familial hemophagocytic lymphohistiocytosis type 5 (FHL-5) syndrome caused by STXBP2/Munc18-2 mutations.  |  Zhao, XW., et al. 2013. Blood. 122: 109-11. PMID: 23687090
12. Associations of functional health literacy with socioeconomic and demographic status among Filipinos.  |  Tejero, LMS., et al. 2022. BMC Public Health. 22: 2198. PMID: 36443735