FHL-2 Antibody (F-1): sc-393514

FHL-2 Antibody (F-1) is a mouse monoclonal IgM antibody that detects FHL-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-FHL-2 antibody (F-1) is available as a non-conjugated format. The four-and-a-half-LIM domain (FHL) proteins, including FHL-1, FHL-2, FHL-3, and FHL-4, are characterized by a unique "half-domain" structure featuring a single zinc finger domain in the N-terminal region, distinguishing FHL proteins from other LIM-only proteins that possess multiple zinc fingers. FHL-2 plays a crucial role in cellular signaling pathways, particularly in selective activation of transcription factors such as CREB and CREM, which are vital for regulating gene expression in response to various stimuli. FHL-2 expression is notably high in cardiac and skeletal muscle tissues, as well as in placenta and ovaries, indicating importance in muscle development and reproductive functions. FHL-2 interaction with other proteins in the FHL family suggests a complex regulatory network influencing muscle differentiation and function. Understanding FHL-2 and associated interactions can provide insights into muscle-related diseases and developmental disorders, making FHL-2 (F-1) monoclonal antibody an essential tool for researchers studying these critical biological processes.

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FHL-2 Antibody (F-1) References:

1. The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle.  |  Morgan, MJ. and Madgwick, AJ. 1999. Biochem Biophys Res Commun. 255: 245-50. PMID: 10049693
2. The fourth member of the FHL family of LIM proteins is expressed exclusively in the testis.  |  Morgan, MJ. and Madgwick, AJ. 1999. Biochem Biophys Res Commun. 255: 251-5. PMID: 10049694
3. Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1.  |  Lee, SM., et al. 1998. Somat Cell Mol Genet. 24: 197-202. PMID: 10226657
4. Genomic structure, tissue expression and chromosomal location of the LIM-only gene, SLIM1.  |  Greene, WK., et al. 1999. Gene. 232: 203-7. PMID: 10352231
5. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.  |  Brown, S., et al. 1999. J Biol Chem. 274: 27083-91. PMID: 10480922
6. A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM.  |  Fimia, GM., et al. 2000. Mol Cell Biol. 20: 8613-22. PMID: 11046156
7. FHL2 Is Essential for Spleen T Cell-Dependent B Cell Activation and Antibody Response.  |  Ebrahimian, T., et al. 2020. Immunohorizons. 4: 259-273. PMID: 32434880
8. Targeting FHL2-E-cadherin axis by miR-340-5p attenuates colon cancer cell migration and invasion.  |  Algaber, A., et al. 2021. Oncol Lett. 22: 637. PMID: 34295384
9. Silencing FHL2 inhibits bleomycin-induced pulmonary fibrosis through the TGF-β1/Smad signaling pathway.  |  Shi, M., et al. 2023. Exp Cell Res. 423: 113470. PMID: 36641135
10. Slim defines a novel family of LIM-proteins expressed in skeletal muscle.  |  Morgan, MJ. and Madgwick, AJ. 1996. Biochem Biophys Res Commun. 225: 632-8. PMID: 8753811
11. Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart.  |  Chan, KK., et al. 1998. Gene. 210: 345-50. PMID: 9573400
12. Chromosomal mapping, tissue distribution and cDNA sequence of four-and-a-half LIM domain protein 1 (FHL1).  |  Lee, SM., et al. 1998. Gene. 216: 163-70. PMID: 9714789

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FHL-2 Antibody (F-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-FHL-2 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FHL-2 Antibody (F-1) Specifications

Alternate Product Name: FHL-2

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 242-270

Epitope Target: FHL-2

Epitope Species: human

Product Reactivity/Detection: FHL-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for FHL-2 Antibody (F-1): P: Blocking Peptide