FHL-1 Antibody (H-4): sc-374246

FHL-1 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects FHL-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FHL-1 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The four-and-a-half-LIM domain (FHL) proteins, which include FHL-1 (also known as SLIM1), play crucial roles in cellular signaling and gene regulation. FHL-1 is particularly important due to its involvement in the selective activation of transcription factors such as CREB and CREM, which are essential for processes like muscle differentiation and cardiac function. FHL-1 is predominantly expressed in skeletal and cardiac muscle, with lower levels found in smooth muscle tissues such as those in the colon, small intestine, and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes, highlighting its significance in muscle development and function. The distinct "half-domain" structure, characterized by a single zinc finger domain in the N-terminal region, sets FHL proteins apart from other LIM-only proteins, which typically contain multiple zinc fingers. This unique structural feature enables specific interactions and regulatory functions of FHL-1 within muscle tissues.

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FHL-1 Antibody (H-4) References:

1. The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle.  |  Morgan, MJ. and Madgwick, AJ. 1999. Biochem Biophys Res Commun. 255: 245-50. PMID: 10049693
2. The fourth member of the FHL family of LIM proteins is expressed exclusively in the testis.  |  Morgan, MJ. and Madgwick, AJ. 1999. Biochem Biophys Res Commun. 255: 251-5. PMID: 10049694
3. Chromosomal mapping of a skeletal muscle specific LIM-only protein FHL3 to the distal end of the short arm of human chromosome 1.  |  Lee, SM., et al. 1998. Somat Cell Mol Genet. 24: 197-202. PMID: 10226657
4. Genomic structure, tissue expression and chromosomal location of the LIM-only gene, SLIM1.  |  Greene, WK., et al. 1999. Gene. 232: 203-7. PMID: 10352231
5. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.  |  Brown, S., et al. 1999. J Biol Chem. 274: 27083-91. PMID: 10480922
6. A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM.  |  Fimia, GM., et al. 2000. Mol Cell Biol. 20: 8613-22. PMID: 11046156
7. Slim defines a novel family of LIM-proteins expressed in skeletal muscle.  |  Morgan, MJ. and Madgwick, AJ. 1996. Biochem Biophys Res Commun. 225: 632-8. PMID: 8753811
8. Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart.  |  Chan, KK., et al. 1998. Gene. 210: 345-50. PMID: 9573400
9. Chromosomal mapping, tissue distribution and cDNA sequence of four-and-a-half LIM domain protein 1 (FHL1).  |  Lee, SM., et al. 1998. Gene. 216: 163-70. PMID: 9714789

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FHL-1 Antibody (H-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-FHL-1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FHL-1 Antibody (H-4) Specifications

Alternate Product Name: FHL-1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-280

Epitope Target: FHL-1

Epitope Species: human

Product Reactivity/Detection: FHL-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for FHL-1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin