FEN-1 Antibody (4E7): sc-56675
- FEN-1 Antibody (4E7) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
- raised against amino acids 1-380 of FEN-1 of human origin
- recommended for detection of FEN-1 of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for FEN-1 Antibody (4E7). This work is in progress.
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FEN-1 Antibody (4E7) is a mouse monoclonal IgG1 antibody that detects FEN-1 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). FEN-1, or flap endonuclease-1, plays a crucial role in maintaining genome stability by participating in DNA replication, recombination, and repair processes. FEN-1 is essential for the proper processing of Okazaki fragments during DNA replication and is involved in excising damaged DNA segments to correct mismatches during repair. Notably, FEN-1 is highly homologous to the yeast RAD2 protein, indicating evolutionary conservation and importance in cellular functions. The C-terminal region of FEN-1 interacts with proliferating cell nuclear antigen (PCNA), which enhances exonuclease activity during DNA replication. This interaction ensures efficient and accurate replication of DNA, thereby preventing genomic instability that can lead to various diseases, including cancer. Anti-FEN-1 antibody (4E7) serves as an invaluable tool for researchers studying DNA repair mechanisms and FEN-1′s implications in cellular processes.
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FEN-1 Antibody (4E7) References:
- Inhibition of FEN-1 processing by DNA secondary structure at trinucleotide repeats. | Spiro, C., et al. 1999. Mol Cell. 4: 1079-85. PMID: 10635332
- Human FEN-1 can process the 5'-flap DNA of CTG/CAG triplet repeat derived from human genetic diseases by length and sequence dependent manner. | Lee, S. and Park, MS. 2002. Exp Mol Med. 34: 313-7. PMID: 12515398
- Preparation and crystallization of human flap endonuclease FEN-1 in complex with proliferating-cell nuclear antigen, PCNA. | Sakurai, S., et al. 2003. Acta Crystallogr D Biol Crystallogr. 59: 933-5. PMID: 12777816
- WRN helicase and FEN-1 form a complex upon replication arrest and together process branchmigrating DNA structures associated with the replication fork. | Sharma, S., et al. 2004. Mol Biol Cell. 15: 734-50. PMID: 14657243
- Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment processing during Okazaki fragment maturation. | Chai, Q., et al. 2003. Biochemistry. 42: 15045-52. PMID: 14690413
- The DNA-protein interaction modes of FEN-1 with gap substrates and their implication in preventing duplication mutations. | Liu, R., et al. 2006. Nucleic Acids Res. 34: 1772-84. PMID: 16582103
- Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human. | Hiraoka, LR., et al. 1995. Genomics. 25: 220-5. PMID: 7774922
- Processing of branched DNA intermediates by a complex of human FEN-1 and PCNA. | Wu, X., et al. 1996. Nucleic Acids Res. 24: 2036-43. PMID: 8668533
- The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21. | Gary, R., et al. 1997. J Biol Chem. 272: 24522-9. PMID: 9305916
- Down-regulation of human FEN-1 gene expression during differentiation of promyelocytic leukemia cells. | Kim, IS. 1998. Exp Mol Med. 30: 252-6. PMID: 9894157
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FEN-1 Antibody (4E7) | sc-56675 | 50 µg/0.5 ml | $322.00 |