Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FcRn Double Nickase Plasmid (m): sc-420308-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FcRn Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FcRn Double Nickase Plasmid (m) and FcRn Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Fcgrt. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FcRn Antibody (A-6): sc-393064
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FcRn Double Nickase Plasmid (m) | sc-420308-NIC | 20 µg | $410.00 | |||
FcRn Double Nickase Plasmid (m2) | sc-420308-NIC-2 | 20 µg | $410.00 |
Mouse Fcgrt encodes the neonatal Fc receptor (FcRn), an MHC class I–related molecule that binds IgG and albumin in a pH-dependent manner to regulate their intracellular trafficking and systemic half-life. FcRn functions prominently in endosomal sorting and recycling pathways, limiting lysosomal degradation and supporting transcytosis across polarized epithelia and endothelium. Through these processes, FcRn influences humoral immunity, antigen handling, and tissue distribution of immunoglobulins, with relevance to inflammatory and autoimmune mechanisms driven by IgG homeostasis. Dysregulated FcRn activity has been associated with altered IgG persistence and immune complex biology, making Fcgrt a useful target for studying Fc-dependent immune regulation in mouse models.
FcRn Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Fcgrt locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Fcgrt. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Fcgrt function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Fcgrt-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.