FAT2 Double Nickase Plasmid (h): sc-402931-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAT2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FAT2 Double Nickase Plasmid (h) and FAT2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FAT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAT2 Antibody (8C5): sc-59985
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAT2 Double Nickase Plasmid (h) | sc-402931-NIC | 20 µg | $410.00 | |||
FAT2 Double Nickase Plasmid (h2) | sc-402931-NIC-2 | 20 µg | $410.00 |
FAT2 encodes an atypical cadherin-like transmembrane protein in the FAT protocadherin family that contributes to cell–cell adhesion and organization of epithelial architecture. Through its large extracellular cadherin repeats and intracellular signaling capacity, FAT2 is linked to regulation of cytoskeletal dynamics and planar cell polarity–associated processes that influence cell shape, migration, and tissue morphogenesis. Altered FAT family function has been associated with disrupted polarity and adhesion states that can affect differentiation programs and the balance between proliferation and contact inhibition. FAT2 has been explored in the context of cancer biology and developmental regulation where changes in adhesion signaling and polarity networks are relevant to disease mechanisms.
FAT2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FAT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FAT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FAT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FAT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.